Central giant cell granulomas of the jaws: phenotype and proliferation-associated markers.
Central giant cell granulomas of the jaws: phenotype and proliferation-associated markers.
复制标题
颌骨中央巨细胞肉芽肿：表型和增殖相关标记。
DOI：
10.1111/j.1600-0714.1997.tb00451.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Regezi
中科院分区：
文献类型：
--
作者：
Miriam O'Mailey;M. Pogrel;Jeffery C. B. Stewart;Rebeka G. Silva;J. Regezi
中央巨细胞肉芽肿（CGCG）是一种起源不明的颌骨肿瘤，通常表现出侵略性，但不可预测的临床过程。本研究的目的是确定似乎对这些肿瘤的生物学行为负责的单核细胞的免疫特征。还测定并比较了临床侵袭性和非侵袭性CGCG中细胞周期中的细胞数。16例侵袭性和12例非侵袭性CGCG用CD 34、CD 68、因子XIIIa、α-平滑肌肌动蛋白、脯氨酰4-羟化酶、Ki-67和p53蛋白的抗体进行免疫组化染色。通过显微镜定量评估确定细胞群体和细胞周期中的细胞数量。CD 34阳性细胞仅限于支持血管。CD 68阳性单核细胞在所有肿瘤中构成了一个小的细胞群体。除了两个例外，很少见到因子XIIIa阳性细胞。α-平滑肌肌动蛋白染色存在于大约一半的肿瘤中，偶尔可见大量阳性细胞。大多数单个核细胞成纤维细胞相关抗原阳性。在侵袭性和非侵袭性肿瘤之间没有检测到表型差异。P53蛋白在CGCG中未出现过表达。Ki-67染色显示只有单个核细胞处于细胞周期中，侵袭性和非侵袭性肿瘤之间无差异。我们的结论是CGCG主要是成纤维细胞（和肌纤维母细胞）肿瘤，其中巨噬细胞似乎发挥次要作用。肿瘤细胞未显示向内皮细胞或巨噬细胞相关树突状细胞（因子XIIIa）分化。侵袭性和非侵袭性肿瘤的细胞表型和细胞周期中的细胞数量相似。
Central giant cell granulomas (CGCGs) are jaw tumors of unknown origin that often exhibit an aggressive, though unpredictable, clinical course. The purpose of this study was to determine the immunoprofile of the mononuclear cells that seem to be responsible for the biologic behavior of these tumors. Numbers of cells in cell cycle were also determined and compared in clinically aggressive and non-aggressive CGCGs. Sixteen aggressive and 12 non-aggressive CGCGs were immunohistochemically stained with antibodies to CD34, CD68, factor XIIIa, alpha-smooth muscle actin, prolyl 4-hydroxylase, Ki-67, and p53 protein. Cell populations and numbers of cells in cell cycle were determined through microscopic quantitative assessment. CD34-positive cells were limited to support vessels. CD68-positive mononuclear cells constituted a small population of cells in all tumors. With two exceptions, factor XIIIa-positive cells were rarely seen. Alpha-smooth muscle actin staining was present in approximately half the tumors, and occasionally large numbers of positive cells were seen. Most mononuclear cells were positive for fibroblast-associated antigen. No phenotypic differences were detected between aggressive and non-aggressive tumors. P53 protein did not appear to be overexpressed in CGCGs. Ki-67 staining showed that only mononuclear cells were in cell cycle, and that there were no differences between aggressive and non-aggressive tumors. We conclude that CGCGs are primarily fibroblastic (and myofibroblastic) tumors in which macrophages appear to play a secondary role. Tumor cells show no differentiation toward endothelial cells or macrophage-related dendrocytes (factor XIIIa). Cellular phenotypes and numbers of cells in cell cycle are similar in both aggressive and non-aggressive tumors.
DOI：
--
发表时间：
1992
期刊：
The American journal of pathology
影响因子：
--
作者：
P. Porter;A. Gown;Steven G. Kramp;M. Coltrera
通讯作者：
P. Porter;A. Gown;Steven G. Kramp;M. Coltrera