The N-glycan of the SCR 2 region is essential for membrane cofactor protein (CD46) to function as a measles virus receptor
The N-glycan of the SCR 2 region is essential for membrane cofactor protein (CD46) to function as a measles virus receptor
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SCR 2 区域的 N-聚糖对于膜辅因子蛋白 (CD46) 作为麻疹病毒受体发挥作用至关重要
作者：
A. Maisner;J. Alvarez;M. Liszewski;D. J. Atkinson;J. Atkinson;G. Herrler
膜辅因子蛋白（MCP）（CD 46）是一种补体调节蛋白，是麻疹病毒的细胞受体。其氨基末端部分由四个短的共有重复序列（SCR）组成，其中三个（SCR 1、SCR 2和SCR 4）携带N-连接寡糖。为了确定三种N-聚糖对MCP作为麻疹病毒受体的功能的重要性，我们建立了稳定表达突变MCP的中国仓鼠卵巢（CHO）细胞系，这些突变MCP缺乏N糖基化的三个基序之一（NQ 1，NQ 2和NQ 4）。在另外的突变体（NQ 1 -2）中，两个糖基化基序被改变，允许仅在SCR 4中添加N-连接的寡糖。用三种不同的测定分析突变MCP作为麻疹病毒受体的能力：（i）麻疹病毒血凝素与固定在硝酸纤维素上的MCP的结合;（ii）麻疹病毒与表达野生型或突变MCP的CHO细胞的结合;和（iii）麻疹病毒对转染的CHO细胞的感染。在所有三个试验中，NQ 2和NQ 1 -2突变体作为麻疹病毒受体的能力被严重削弱。NQ 1和NQ 4突变体几乎与野生型蛋白一样有效地被麻疹病毒识别。这些结果表明，连接到SCR 2的N-聚糖是必需的MCP作为麻疹病毒受体，而连接到SCR 1和SCR 4的寡糖是次要的。
Membrane cofactor protein (MCP) (CD46), a complement-regulatory protein, serves as a cellular receptor for measles virus. Its amino-terminal portion is composed of four short consensus repeats (SCR), three of which (SCR1, SCR2, and SCR4) carry an N-linked oligosaccharide. In order to determine the importance of the three N-glycans for the function of MCP as a measles virus receptor, we established Chinese hamster ovary (CHO) cell lines that stably express mutant MCPs lacking one of the three motifs for N glycosylation (NQ1, NQ2, and NQ4). In an additional mutant (NQ1-2), two glycosylation motifs were altered, allowing the addition of an N-linked oligosaccharide only in SCR4. The abilities of the mutant MCPs to function as measles virus receptors were analyzed with three different assays: (i) binding of measles virus hemagglutinin to MCP immobilized on nitrocellulose; (ii) binding of measles virus to CHO cells expressing wild-type or mutant MCP; and (iii) infection of the transfected CHO cells by measles virus. In all three assays, the abilities of the NQ2 and NQ1-2 mutants to serve as measles virus receptors were drastically impaired. The NQ1 and NQ4 mutants were recognized by measles virus almost as efficiently as the wild-type protein. These results indicate that the N-glycan attached to SCR2 is essential for MCP to serve as a measles virus receptor, while the oligosaccharides attached to SCR1 and SCR4 are of only minor importance.
DOI：
10.1073/pnas.91.13.6245
发表时间：
1994-06-21
影响因子：
11.1
作者：
BERGELSON, JM;CHAN, M;FINBERG, RW
通讯作者：
FINBERG, RW
CD46（膜辅因子蛋白）的多种亚型可作为麻疹病毒的受体。
DOI：
10.1073/pnas.91.6.2161
发表时间：
1994
影响因子：
11.1
作者：
Manchester,M;Liszewski,MK;Atkinson,JP;Oldstone,MB
通讯作者：
Oldstone,MB