NDW, encoding a receptor-like protein kinase, regulates plant growth, cold tolerance and susceptibility to Botrytis cinerea in tomato.
NDW, encoding a receptor-like protein kinase, regulates plant growth, cold tolerance and susceptibility to Botrytis cinerea in tomato.
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NDW 编码一种受体样蛋白激酶，可调节番茄的植物生长、耐寒性和对灰霉病菌的敏感性。
DOI：
10.1016/j.plantsci.2020.110684
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发表时间：
2020-09
期刊：
影响因子：
5.2
通讯作者：
Ye Zhibiao
中科院分区：
文献类型：
--
作者：
Xu Wei;Gao Shenghua;Song Jianwen;Yang Qihong;Wang Taotao;Zhang Yuyang;Zhang Junhong;Li Hanxia;Yang Changxian;Ye Zhibiao
植物利用不同的机制来响应和适应不断变化的环境因素。受体样蛋白激酶（Receptor-like protein kinases，RLK）是植物跨膜信号蛋白中最大的家族之一，在植物的生长发育和逆境应答中发挥着重要的作用。在这里，我们确定了坏死性矮化（ndw）突变体渐渗系（IL）6-2，表现出发育迟缓，叶片卷曲，并在低温下进行性坏死。基于图位克隆和转基因分析，我们确定ndw突变体的表型是由编码RLK的NDW表达减少引起的。NDW是一种定位于细胞质膜和细胞质的蛋白质。NDW基因的过表达可以恢复IL 6 -2在低温下的半矮秆和坏死表型，进一步发现NDW基因可以显著降低对灰葡萄孢的敏感性。相反，在M82植株中敲低NDW可增加对B的敏感性。灰叶此外，转录表达分析表明，NDW影响脱落酸（阿坝）信号通路相关基因的表达。这些结果表明，NDW在调节植物生长、耐冷性和减轻对灰葡萄孢的敏感性方面起着重要作用。
Plants utilize different mechanisms to respond and adapt to continuously changing environmental factors. Receptor-like protein kinases (RLKs) comprise one of the largest families of plant transmembrane signaling proteins, which play critical and diverse roles in plant growth, development, and stress response. Here, we identified the necrotic dwarf (ndw) mutant introgression line (IL) 6-2, which demonstrated stunting, leaf curl, and progressive necrosis at low temperatures. Based on map-based cloning and transgenic analysis, we determined that the phenotype of ndw mutant is caused by decreased expression of NDW, which encodes an RLK. NDW is a plasma membrane and cytoplasmic located protein. Overexpression of NDW can restore both of the semi-dwarf and necrotic phenotype in IL6-2 at low temperatures, further we found that NDW could significantly reduce susceptibility to Botrytis cinerea. On the contrary, knockdown NDW in M82 plants could increase the sensitivity to B. cinerea. Furthermore, transcriptional expression analysis showed that NDW affects the expression of genes related to the abscisic acid (ABA) signaling pathway. Taken together, these results indicate that NDW plays an important role in regulating plant growth, cold tolerance and mitigating susceptibility to Botrytis cinerea.
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影响因子：
4.8
作者：
Osakabe, Yuriko;Mizuno, Shinji;Yamaguchi-Shinozaki, Kazuko
通讯作者：
Yamaguchi-Shinozaki, Kazuko
影响因子：
5.6
作者：
Lim CW;Baek W;Jung J;Kim JH;Lee SC
通讯作者：
Lee SC
影响因子：
1.4
作者：
N. I. Vasyukova;O. L. Ozeretskovskaya
通讯作者：
N. I. Vasyukova;O. L. Ozeretskovskaya
DOI：
--
发表时间：
2004
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
J. Ton;B. Mauch-Mani
通讯作者：
J. Ton;B. Mauch-Mani
ShDHN（来自 Solanum habrochaites 的脱水蛋白基因）的过度表达可增强番茄对多种非生物胁迫的耐受性
影响因子：
5.2
作者：
Liu, Hui;Yu, Chuying;Ye, Zhibiao
通讯作者：
Ye, Zhibiao