Exocyst complex protein expression in the human placenta.
Exocyst complex protein expression in the human placenta.
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DOI：
10.1016/j.placenta.2014.04.015
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发表时间：
2014-07
期刊：
影响因子：
3.8
通讯作者：
Robinson, J. M.
中科院分区：
文献类型：
--
作者：
Gonzalez, I. M.;Ackerman, W. E.;Vandre, D. D.;Robinson, J. M.
蛋白质的产生和分泌对于合胞体滋养层功能至关重要，并且与细胞滋养层细胞融合和分化相关。合体滋养层激素分泌是母胎健康的关键决定因素，在病理妊娠中可能失调。虽然，极化分泌是胎盘功能的关键组成部分，但对这一过程的机制知之甚少。虽然八聚体外囊复合物被经典地认为是各种哺乳动物系统中分泌的主要调节剂，但其在胎盘中的表达仍未被探索。我们假设，合体滋养层将表达囊泡介导的分泌所需的所有外囊复合物成分和效应蛋白比组织标本中的细胞滋养层更丰富。一个两层的免疫生物学方法被用来表征正常的，长期的人类胎盘外囊和辅助蛋白。通过免疫印迹和免疫荧光标记胎盘切片，在组织匀浆中记录外囊蛋白表达和定位。在人胎盘中发现了8种外囊蛋白EXOC 1、2、3、4、5、6、7和8。此外，还表达了一种重要的外囊复合物调节剂RAB11。外囊和Rab蛋白的表达似乎在滋养层分化过程中受到调节，因为合胞体滋养层表达这些蛋白，但在细胞滋养层细胞中表达很少（如果有的话）。此外，exocyst蛋白定位在或附近的合胞体滋养层顶膜，胎盘分泌的主要网站我们的研究结果突出exocyst蛋白的表达作为滋养层分化的新指标。外囊在合胞体滋养层内的调节定位及其众所周知的功能提示其可能在胎盘极化分泌中起作用
Protein production and secretion are essential to syncytiotrophoblast function and are associated with cytotrophoblast cell fusion and differentiation. Syncytiotrophoblast hormone secretion is a crucial determinant of maternal-fetal health, and can be misregulated in pathological pregnancies. Although, polarized secretion is a key component of placental function, the mechanisms underlying this process are poorly understood. While the octameric exocyst complex is classically regarded as a master regulator of secretion in various mammalian systems, its expression in the placenta remained unexplored. We hypothesized that the syncytiotrophoblast would express all exocyst complex components and effector proteins requisite for vesicle-mediated secretion more abundantly than cytotrophoblasts in tissue specimens. A two-tiered immunobiological approach was utilized to characterize exocyst and ancillary proteins in normal, term human placentas. Exocyst protein expression and localization was documented in tissue homogenates via immunoblotting and immunofluorescence labeling of placental sections. The eight exocyst proteins, EXOC1, 2, 3, 4, 5, 6, 7, and 8, were found in the human placenta. In addition, RAB11, an important exocyst complex modulator, was also expressed. Exocyst and Rab protein expression appeared to be regulated during trophoblast differentiation, as the syncytiotrophoblast expressed these proteins with little, if any, expression in cytotrophoblast cells. Additionally, exocyst proteins were localized at or near the syncytiotrophoblast apical membrane, the major site of placental secretion Our findings highlight exocyst protein expression as novel indicators of trophoblast differentiation. The exocyst’s regulated localization within the syncytiotrophoblast in conjunction with its well known functions suggests a possible role in placental polarized secretion
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DOI：
10.1523/jneurosci.3907-09.2009
发表时间：
2009-10-21
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dupraz S;Grassi D;Bernis ME;Sosa L;Bisbal M;Gastaldi L;Jausoro I;Cáceres A;Pfenninger KH;Quiroga S
通讯作者：
Quiroga S
影响因子：
64.5
作者：
NOVICK, P;FIELD, C;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
3.8
作者：
Redman, C. W. G.;Sargent, I. L.
通讯作者：
Sargent, I. L.
酵母CDC42在胞吐作用的后期发挥作用，特别是在新兴芽的极化生长过程中。
影响因子：
7.8
作者：
Adamo, JE;Moskow, JJ;Gladfelter, AS;Viterbo, D;Lew, DJ;Brennwald, PJ
通讯作者：
Brennwald, PJ
影响因子：
4.8
作者：
Baek, Kyuwon;Knoedler, Andreas;Dominguez, Roberto
通讯作者：
Dominguez, Roberto