Selective changes in the neurofilament and microtubule cytoskeleton of NF‐H/LacZ mice
Selective changes in the neurofilament and microtubule cytoskeleton of NF‐H/LacZ mice
复制标题
NF-H/LacZ 小鼠神经丝和微管细胞骨架的选择性变化
DOI：
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发表时间：
2003
影响因子：
4.2
通讯作者：
B. Riederer
中科院分区：
文献类型：
--
作者：
I. Riederer;P. Robert;R. Porchet;J. Eyer;B. Riederer
This study focused mainly on changes in the microtubule cytoskeleton in a transgenic mouse where β‐galactosidase fused to a truncated neurofilament subunit led to a decrease in neurofilament triplet protein expression and a loss in neurofilament assembly and abolished transport into neuronal processes in spinal cord and brain. Although all neurofilament subunits accumulated in neuronal cell bodies, our data suggest an increased solubility of all three subunits, rather than increased precipitation, and point to a perturbed filament assembly. In addition, reduced neurofilament phosphorylation may favor an increased filament degradation. The function of microtubules seemed largely unaffected, in that tubulin and microtubule‐associated proteins (MAP) expression and their distribution were largely unchanged in transgenic animals. MAP1A was the only MAP with a reduced signal in spinal cord tissue, and differences in immunostaining in various brain regions corroborate a relationship between MAP1A and neurofilaments. © 2002 Wiley‐Liss, Inc.
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使用紫杉醇纯化脑微管和微管相关蛋白 1。
影响因子：
--
作者：
Vallee,RB
通讯作者：
Vallee,RB
使用结构域交换嵌合蛋白分析 IV 型大鼠神经元中间丝蛋白的头结构域在丝组装中的作用。
影响因子：
4
作者：
Ching,GY;Liem,RK
通讯作者：
Liem,RK
DOI：
10.1002/neu.480180205
发表时间：
1987
期刊：
Journal of neurobiology
影响因子：
--
作者：
Glicksman,MA;Soppet,D;Willard,MB
通讯作者：
Willard,MB
影响因子：
2.9
作者：
Tokutake,S;Hutchison,SB;Pachter,JS;Liem,RK
通讯作者：
Liem,RK