Post-transcriptional regulation of plasminogen activator inhibitor type-1 expression in human pleural mesothelial cells.
Post-transcriptional regulation of plasminogen activator inhibitor type-1 expression in human pleural mesothelial cells.
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人胸膜间皮细胞中纤溶酶原激活剂抑制剂 1 型表达的转录后调节。
DOI：
10.1165/rcmb.2009-0046oc
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发表时间：
2010
影响因子：
6.4
通讯作者：
Idell,Steven
中科院分区：
文献类型：
--
作者：
Shetty,Sreerama;Velusamy,Thirunavukkarasu;Shetty,RashmiS;Marudamuthu,AmarnathS;Shetty,ShwethaK;Florova,Galina;Tucker,Torry;Koenig,Kathy;Shetty,Praveenkumar;Bhandary,YashodharP;Idell,Steven
The plasminogen activator inhibitor type–1 (PAI-1) effectively blocks the activities of free and receptor-bound urokinase-type plasminogen activator. Incubation of cultured human pleural mesothelial (Met5A) cells with TGF-β increased PAI-1 protein. TGF-β, phorbol myristate acetate, and the translation inhibitor cycloheximide induced PAI-1 mRNA and slowed its degradation, suggesting that PAI-1 mRNA could be regulated by interaction of a PAI-1 binding protein (PAI-1 mRNABp) with PAI-1 mRNA. We found that an approximately 60 kD cytoplasmic PAI-1 mRNABp is detectable in cytoplasmic extracts of MeT5A human pleural mesothelial and malignant mesothelioma cells. The PAI-1 mRNABp specifically binds to a 33-nt sequence in the 3′ untranslated region of PAI-1 mRNA. Insertion of this 33-nt sequence destabilizes otherwise stable β-globin mRNA, indicating that the binding sequence accelerates decay of endogenous PAI-1 mRNA. Competitive inhibition by overexpression of the 33-nt binding sequence in MeT5A cells reduced PAI-1 mRNA decay and increased PAI-1 protein and mRNA expression, indicating that the PAI-1 mRNABp destabilizes PAI-1 mRNA by its interaction with the endogenous 33-nt binding sequence. Incubation of Met5A cells with TGF-β attenuated the interaction of the PAI-1 mRNABp with the 33-nt sequence. By conventional and affinity purification, we isolated the PAI-1 mRNABp and confirmed its identity as 6-phospho-d-gluconate-NADP oxidoreductase, which specifically interacts with the full-length and the 33-nt sequence of the PAI-1 mRNA 3′ untranslated region. This newly recognized pathway could influence expression of PAI-1 by mesothelial or mesothelioma cells at the level of mRNA stability in the context of pleural inflammation or malignancy.
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在人横纹肌肉瘤细胞中，佛波酯可增加纤溶酶原激活剂抑制剂 1 型蛋白、mRNA 和基因转录。
DOI：
10.1016/s0021-9258(19)37642-2
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Mayer;L. Lund;A. Riccio;J. Skouv;L. Nielsen;S. Stacey;K. Danø;P. Andreasen
通讯作者：
P. Andreasen
TGF-β1 诱导的 PAI-1 基因表达需要 MEK 活性和细胞与基质的粘附。
影响因子：
4
作者：
Kutz,SM;Hordines,J;McKeown-Longo,PJ;Higgins,PJ
通讯作者：
Higgins,PJ
佛波酯在人肝癌细胞中对两种纤溶酶原激活剂抑制剂 1 mRNA 种类的不同诱导。
DOI：
10.1016/s0021-9258(18)55205-4
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Bosma;T. Kooistra
通讯作者：
T. Kooistra
影响因子：
8.8
作者：
Kozar, RA;Weibel, CJ;Trooskin, SZ
通讯作者：
Trooskin, SZ
单链尿激酶在兔四环素诱导的胸膜损伤中的胸膜内激活、处理、功效和保护持续时间。
DOI：
10.1152/ajplung.00118.2006
发表时间：
2007
期刊：
American journal of physiology. Lung cellular and molecular physiology
影响因子：
--
作者：
Idell,Steven;Allen,Timothy;Chen,Shande;Koenig,Kathy;Mazar,Andrew;Azghani,Ali
通讯作者：
Azghani,Ali