Genome-wide response to selection and genetic basis of cold tolerance in rice (Oryza sativa L.).
Genome-wide response to selection and genetic basis of cold tolerance in rice (Oryza sativa L.).
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水稻（Oryza sativa L.）耐寒性选择和遗传基础的全基因组响应
DOI：
10.1186/1471-2156-15-55
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发表时间：
2014-05-08
期刊：
影响因子：
2.9
通讯作者：
Li ZK
中科院分区：
文献类型：
--
作者：
Zhang F;Ma XF;Gao YM;Hao XB;Li ZK
冷胁迫是许多高纬度高海拔地区限制水稻产量的重要因素。利用DNA分子标记对水稻耐冷性进行遗传分析已经取得了相当大的进展。由于数量性状基因座可能存在上位性和基因与环境互作，这些遗传研究的结果不能直接应用于改良水稻CT的分子标记辅助选择。在这项研究中，我们证明了实用的选择性渐渗策略，同时改善和遗传解剖的水稻幼苗CT.ResultsA组粳稻渐渗系（ILs）显着改善幼苗CT开发了四个回交群体的基础上，两轮的选择。这些耐寒IL的遗传特性揭示了水稻CT强表型选择的两个重要方面：（1）在整个水稻基因组的29个功能遗传单位（FGU）的57个位点上，供体等位基因的显着过度渐渗和（2）在许多非连锁CT位点的等位基因之间或之间的显着非随机关联。连锁不平衡分析检测到的CT位点，使我们能够构建推定的遗传网络（多位点结构）的水稻幼苗CT。每个网络由一个单一的FGU，高基因渗入作为推定的调节剂加上两到三组高度相关的下游FGU。一个生物信息学搜索的水稻基因组区域窝藏这些公认的监管机构确定了一个小的候选人的调控基因，已知参与plant stress respons.ConclusionsOur结果表明，CT在水稻是由多个途径控制。
BackgroundCold stress is an important factor limiting rice yield in many areas of high latitude and altitude. Considerable efforts have been taken to genetically dissect cold tolerance (CT) in rice using DNA markers. Because of possible epistasis and gene × environment interactions associated with identified quantitative trait loci, the results of these genetic studies have unfortunately not been directly applicable to marker-assisted selection for improved rice CT. In this study, we demonstrated the utility of a selective introgression strategy for simultaneous improvement and genetic dissection of rice seedling CT.ResultsA set of japonica introgression lines (ILs) with significantly improved seedling CT were developed from four backcross populations based on two rounds of selection. Genetic characterization of these cold-tolerant ILs revealed two important aspects of genome-wide responses to strong phenotypic selection for rice CT: (1) significant over-introgression of donor alleles at 57 loci in 29 functional genetic units (FGUs) across the rice genome and (2) pronounced non-random associations between or among alleles at many unlinked CT loci. Linkage disequilibrium analyses of the detected CT loci allowed us to construct putative genetic networks (multi-locus structures) underlying the seedling CT of rice. Each network consisted of a single FGU, with high introgression as the putative regulator plus two to three groups of highly associated downstream FGUs. A bioinformatics search of rice genomic regions harboring these putative regulators identified a small set of candidate regulatory genes that are known to be involved in plant stress response.ConclusionsOur results suggest that CT in rice is controlled by multiple pathways.
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影响因子：
2.4
作者：
Iwata, Natsuko;Shinada, Hiroshi;Fujino, Kenji
通讯作者：
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