A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems.
A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems.
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一种高度特异性的比率双光子荧光探针，用于检测血浆和生命系统中的二肽基肽酶 IV
DOI：
10.1016/j.bios.2016.11.068
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发表时间：
2017-04-15
影响因子：
12.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Zou LW;Wang P;Qian XK;Feng L;Yu Y;Wang DD;Jin Q;Hou J;Liu ZH;Ge GB;Yang L
在本研究中，开发并充分表征了一种高特异性的比率型双光子荧光探针GP - BAN，用于监测血浆和生命体系中的二肽基肽酶IV。GP - BAN是基于二肽基肽酶IV（DPP - IV）的催化特性和底物偏好设计的，在生理条件下加入DPP - IV时，它能轻易地发生水解。反应表型分析和抑制试验均表明，与其他人类丝氨酸水解酶（包括成纤维细胞激活蛋白（FAP）、二肽基肽酶VIII（DPP - VIII）和二肽基肽酶IX（DPP - IX））相比，GP - BAN对DPP - IV表现出良好的反应性和高选择性。该探针成功用于监测包括稀释血浆在内的复杂生物体系中DPP - IV的实际活性，同时，以人血浆或组织提取物作为酶源，它可用于DPP - IV抑制剂的高通量筛选。作为一种双光子荧光探针，GP - BAN还成功用于活细胞和组织中内源性DPP - IV的双光子成像，并展现出高比率成像分辨率和深层组织穿透能力。综上所述，开发并充分表征了一种比率型双光子荧光探针GP - BAN，用于在复杂生物体系中高选择性、灵敏地检测DPP - IV，它有望成为一种有前景的成像工具，用于探究这种关键酶在生命体系中的生物学功能和生理作用。
已开发出一种高特异性双光子荧光探针GP - BAN。
该探针成功应用于检测复杂生物样品中的DPP - IV。
该探针可用于DPP - IV抑制剂的高通量筛选。
该探针成功用于活细胞和组织中DPP - IV的双光子成像。
In this study, a highly specific ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized to monitor dipeptidyl peptidase IV in plasma and living systems. GP-BAN was designed on the basis of the catalytic properties and substrate preference of DPP-IV, and it could be readily hydrolyzed upon addition of DPP-IV under physiological conditions. Both reaction phenotyping and inhibition assays demonstrated that GP-BAN displayed good reactivity and high selectivity towards DPP-IV over other human serine hydrolases including FAP, DPP-VIII, and DPP-IX. The probe was successfully used to monitor the real activities of DPP-IV in complex biological systems including diluted plasma, while it could be used for high throughput screening of DPP-IV inhibitors by using human plasma or tissue preparations as enzyme sources. As a two-photon fluorescent probe, GP-BAN was also successfully used for two-photon imaging of endogenous DPP-IV in living cells and tissues, and showed high ratiometric imaging resolution and deep-tissue penetration ability. Taken together, a ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized for highly selective and sensitive detection of DPP-IV in complex biological systems, which could serve as a promising imaging tool to explore the biological functions and physiological roles of this key enzyme in living systems. A highly specific two-photon fluorescent probe GP-BAN has been developed. The probe was successfully applied to detect DPP-IV in complex biological samples. The probe could be used for high throughput screening of DPP-IV inhibitors. The probe was successfully used for two-photon imaging of DPP-IV in living cells and tissues.
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Somogyi A
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