The microRNA expression profile of mouse Müller glia in vivo and in vitro.
The microRNA expression profile of mouse Müller glia in vivo and in vitro.
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DOI：
10.1038/srep35423
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发表时间：
2016-10-14
影响因子：
4.6
通讯作者：
Reh TA
中科院分区：
文献类型：
--
作者：
Wohl SG;Reh TA
成熟神经胶质细胞中的miRNAs谱尚未得到很好的表征，大多数研究都是在培养的神经胶质细胞中进行的。为了鉴定神经视网膜的成年和年轻（出生后第11/12天）Müller胶质细胞中的miRNA，我们通过荧光激活细胞分选从Rlbp-CreER：Stopf/f-tdTomato小鼠分离Müller胶质细胞，并使用NanoStrings Technologies®分析其miRNA。在新鲜分离的成年Müller胶质细胞中，我们鉴定了7种在胶质细胞中高表达水平，但在视网膜神经元中表达水平非常低的miRNA。这些包括miR-204、miR-9和miR-125- 5 p。我们还发现了15种在神经元和胶质细胞中高水平表达的miRNA，以及许多在神经元中富集并在Müller胶质细胞中以较低水平表达的miRNA，如miR-124。接下来，我们比较了急性分离的Müller神经胶质细胞的miRNA表达与在分离培养物中维持8天和14天的细胞的miRNA表达。我们发现，大多数miRNAs在体外下降。有趣的是，一些在成年Müller胶质细胞中不高表达的miRNA在培养细胞中增加。因此，我们的研究结果显示了成年Müller胶质细胞的miRNA谱以及细胞培养对其水平的影响。
The profile of miRNAs in mature glia is not well characterized, and most studies have been done in cultured glia. In order to identify the miRNAs in adult and young (postnatal day 11/12) Müller glia of the neural retina, we isolated the Müller glia from Rlbp-CreER: Stopf/f-tdTomato mice by means of fluorescent activated cell sorting and analyzed their miRNAs using NanoStrings Technologies®. In freshly isolated adult Müller glia, we identified 7 miRNAs with high expression levels in the glia, but very low levels in the retinal neurons. These include miR-204, miR-9, and miR-125–5p. We also found 15 miRNAs with high levels of expression in both neurons and glia, and many miRNAs that were enriched in neurons and expressed at lower levels in Müller glia, such as miR-124. We next compared miRNA expression of acutely isolated Müller glia with those that were maintained in dissociated culture for 8 and 14 days. We found that most miRNAs declined in vitro. Interestingly, some miRNAs that were not highly expressed in adult Müller glia increased in cultured cells. Our results thus show the miRNA profile of adult Müller glia and the effects of cell culture on their levels.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
5.1
作者：
Chu YY;Ko CY;Wang WJ;Wang SM;Gean PW;Kuo YM;Wang JM
通讯作者：
Wang JM
影响因子：
5.2
作者：
Krichevsky, Anna M.;Sonntag, Kai-C.;Kosik, Kenneth S.
通讯作者：
Kosik, Kenneth S.
影响因子：
4.4
作者：
Karali, Marianthi;Peluso, Ivana;Banfi, Sandro
通讯作者：
Banfi, Sandro
DOI：
10.1073/pnas.0811523106
发表时间：
2009-04-28
影响因子：
11.1
作者：
Hwang, Hun-Way;Wentzel, Erik A.;Mendell, Joshua T.
通讯作者：
Mendell, Joshua T.