PI4P and BLOC-1 remodel endosomal membranes into tubules.
PI4P and BLOC-1 remodel endosomal membranes into tubules.
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DOI：
10.1083/jcb.202110132
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发表时间：
2022-11-07
影响因子：
7.8
通讯作者：
Delevoye, Cedric
中科院分区：
文献类型：
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作者：
Jani, Riddhi Atul;Di Cicco, Aurelie;Keren-Kaplan, Tal;Vale-Costa, Silvia;Hamaoui, Daniel;Hurbain, Ilse;Tsai, Feng-Ching;Di Marco, Mathilde;Mace, Anne-Sophie;Zhu, Yueyao;Amorim, Maria Joao;Bassereau, Patricia;Bonifacino, Juan S.;Subtil, Agathe;Marks, Michael S.;Levy, Daniel;Raposo, Graca;Delevoye, Cedric
管状再循环内体源自早期内体以将内容物递送至靶膜。Jani等人表明，BLOC-1和PI 4P（由PI 4KII合成）重塑膜以产生和稳定用于病原体运输和利用的再循环小管。细胞内运输是由来源于供体细胞器的膜重塑的运输载体介导的。管状载体有助于膜脂质和蛋白质向受体细胞器的通量，但脂质和蛋白质如何在载体上施加管状几何形状尚不完全清楚。使用细胞和体外膜系统的成像方法，我们表明，磷脂酰肌醇-4-磷酸（PI 4P）和溶酶体相关的细胞器复合物1（BLOC-1）的生物发生支配的形成，稳定性和功能的回收内体小管。在体外，BLOC-1结合并微管化带负电荷的膜，包括含有PI 4P的膜。在细胞中，需要II型PI 4激酶产生内体PI 4 P来形成和稳定BLOC-1依赖性再循环内体小管。降低的PI 4KII表达损害内体货物的再循环和利用再循环内体的膜动力学的细胞内病原体（例如衣原体细菌和流感病毒）的生命周期。这项研究展示了磷脂和蛋白质复合物如何协调细胞膜重塑为功能性小管。
Tubular recycling endosomes originate from early endosomes to deliver contents to target membranes. Jani et al. show that BLOC-1 and PI4P (synthesized by PI4KIIs) remodel membranes to generate and stabilize recycling tubules for cargo trafficking and exploitation by pathogens. Intracellular trafficking is mediated by transport carriers that originate by membrane remodeling from donor organelles. Tubular carriers contribute to the flux of membrane lipids and proteins to acceptor organelles, but how lipids and proteins impose a tubular geometry on the carriers is incompletely understood. Using imaging approaches on cells and in vitro membrane systems, we show that phosphatidylinositol-4-phosphate (PI4P) and biogenesis of lysosome-related organelles complex 1 (BLOC-1) govern the formation, stability, and functions of recycling endosomal tubules. In vitro, BLOC-1 binds and tubulates negatively charged membranes, including those containing PI4P. In cells, endosomal PI4P production by type II PI4-kinases is needed to form and stabilize BLOC-1-dependent recycling endosomal tubules. Decreased PI4KIIs expression impairs the recycling of endosomal cargoes and the life cycles of intracellular pathogens such as Chlamydia bacteria and influenza virus that exploit the membrane dynamics of recycling endosomes. This study demonstrates how a phospholipid and a protein complex coordinate the remodeling of cellular membranes into functional tubules.
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影响因子：
11.8
作者：
Chang-Ileto, Belle;Frere, Samuel G.;Chan, Robin B.;Voronov, Sergey V.;Roux, Aurelian;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
影响因子：
5.4
作者：
Amorim, Maria Joao;Bruce, Emily A.;Digard, Paul
通讯作者：
Digard, Paul
影响因子：
3.4
作者：
Allgood SC;Neunuebel MR
通讯作者：
Neunuebel MR
影响因子：
64.5
作者：
Dippold HC;Ng MM;Farber-Katz SE;Lee SK;Kerr ML;Peterman MC;Sim R;Wiharto PA;Galbraith KA;Madhavarapu S;Fuchs GJ;Meerloo T;Farquhar MG;Zhou H;Field SJ
通讯作者：
Field SJ
影响因子：
21.3
作者：
通讯作者：
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