Effects of Intravenously Administered β‐Human Atrial Natriuretic Polypeptide in Humans
Effects of Intravenously Administered β‐Human Atrial Natriuretic Polypeptide in Humans
复制标题
静脉注射β-人心房钠尿多肽对人体的作用
作者：
H. Itoh;M. Mukoyama;S. Shiono;N. Morii;A. Sugawara;Takayuki Yamada;Yoshihiko Saito;H. Arai;Klminori Hosoda;Y. Kambayashi;K. Inouye;H. Imura
β-人心钠素(β-hANP)是从人心房中分离到的α-人心钠素(a-hANP)的反平行二聚体。利用人工合成的β-hANP和α-hANP的放射免疫法(同时检测β-hANP)，我们先前已经证明在体外人血浆中β-hANP被转化为α-hANP。在本研究中，我们比较了5名正常人静脉注射β-hANP(100μg)和等摩尔注射α-hANP(50μg)的效果，并探讨了β-hANP的可能作用机制。虽然α-hANP引起血压显著降低，但β-hANP引起的血压变化很小。相反，β-hANP比α-hANP具有更强、更持久的利尿和利钠活性。β-hANP引起的尿量和钠排泄净变化(579±65ml，56.0±9.9mEq)明显大于α-hANP(396±50ml，34.7±4.9mEq；p<；0.05)。β-hANP组较α-hANP组血浆心钠素样免疫反应性保留时间更长。高效凝胶渗透色谱结合放射免疫分析表明，β-hANP(AFR6000)在人血浆中也可转化为α-hANP(Mr=3000)。β-hANP向α-hANP的转化可能与β-hANP在人体内观察到的效果有关。
β-Human atrial natriuretic polypeptide (β-hANP) is an antiparallel dimer of α-human ANP (a-hANP) that was isolated from human atria. Using synthetic β-hANP and a radioimmunoassay for α-hANP that also detects β-hANP, we have previously demonstrated that β-hANP is converted into α-hANP in human plasma in vitro. In the present study, we compared the effects of intravenous administration of β-hANP (100 μg) to five normal human volunteers with those of an equimolar administration of α-hANP (50 μg) to the same subjects, and we also investigated the possible mechanisms of actions of β-hANP. Although the administration of α-hANP caused a significant decrease in blood pressure with a reactional increase of heart rate, β-hANP elicited minimal change of blood pressure. In contrast, β-hANP exerted more potent and longer lasting diuretic and natriuretic activities than did α-hANP. Net changes in urine volume and sodium excretion induced by β-hANP (579 ± 65 ml, 56.0 ± 9.9 mEq) were significantly greater than those elicited by α-hANP (396 ± 50 ml, 34.7 ± 4.9 mEq; p < 0.05, respectively). The administration ofβ-hANP revealed a longer retention of the ANP-like immunoreactivity level in plasma, compared with that of α-hANP. High performance gel permeation chromatography coupled with the radioimmunoassay revealed that β-hANP (Afr=6000) was also converted into α-hANP (Mr=3000) in human plasma in vivo. The demonstrated conversion of β-hANP into α-hANP could be relevant to the observed effects of β-hANP in humans.
肾血流动力学与心房利尿钠因子利钠作用的关系。
DOI：
10.1152/ajprenal.1986.250.3.f520
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Sosa,RE;Volpe,M;Marion,DN;Atlas,SA;Laragh,JH;VaughanJr,ED;Maack,T
通讯作者：
Maack,T