Characterization of CIPK Family in Asian Pear (Pyrus bretschneideri Rehd) and Co-expression Analysis Related to Salt and Osmotic Stress Responses.
Characterization of CIPK Family in Asian Pear (Pyrus bretschneideri Rehd) and Co-expression Analysis Related to Salt and Osmotic Stress Responses.
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亚洲梨 (Pyrus bretschneideri Behd) CIPK 家族的特征以及与盐和渗透胁迫反应相关的共表达蛋白分析
DOI：
10.3389/fpls.2016.01361
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chang Y
中科院分区：
文献类型：
--
作者：
Tang J;Lin J;Li H;Li X;Yang Q;Cheng ZM;Chang Y
亚洲梨（Pyrus bretschneideri）是世界上最重要的水果作物之一，其生长和产量经常受到非生物胁迫的影响。钙调磷酸酶b样相互作用蛋白激酶（CIPKs）作为钙传感器蛋白激酶与Ca2+结合的CBLs相互作用，广泛介导植物的非生物胁迫反应。虽然梨基因组序列已经公布，但关于梨CIPK基因的信息很少，特别是对盐和渗透胁迫的反应。在这项研究中，我们系统地从梨基因组中鉴定了28个CIPK家族成员，并分析了它们的组织、系统发育、基因结构、蛋白质基序和共链重复差异。大多数PbCIPKs都经历了纯化选择，它们的进化分化伴随着梨全基因组的重复。我们还研究了盐胁迫和渗透胁迫下CIPK家族的胁迫响应表达模式和共表达网络，以及启动子区域中与胁迫相关的顺式调控元件的分布。我们的研究结果表明，大多数PbCIPKs可能在非生物胁迫响应中发挥重要作用。基于pbcipk共表达网络，PbCIPK22、19、-18、-15、-8和-6等pbcipk可作为盐胁迫和渗透胁迫响应的核心调控因子。一些参与盐反应的基因组与渗透反应的基因组没有重叠，这表明CIPK基因在应激反应中具有亚功能化。本研究揭示了一些在盐胁迫和渗透胁迫早期应答中发挥作用的候选基因，为进一步表征梨CIPKs介导的非生物胁迫应答提供了依据。
Asian pear (Pyrus bretschneideri) is one of the most important fruit crops in the world, and its growth and productivity are frequently affected by abiotic stresses. Calcineurin B-like interacting protein kinases (CIPKs) as caladium-sensor protein kinases interact with Ca2+-binding CBLs to extensively mediate abiotic stress responses in plants. Although the pear genome sequence has been released, little information is available about the CIPK genes in pear, especially in response to salt and osmotic stresses. In this study, we systematically identified 28 CIPK family members from the sequenced pear genome and analyzed their organization, phylogeny, gene structure, protein motif, and synteny duplication divergences. Most duplicated PbCIPKs underwent purifying selection, and their evolutionary divergences accompanied with the pear whole genome duplication. We also investigated stress -responsive expression patterns and co-expression networks of CIPK family under salt and osmotic stresses, and the distribution of stress-related cis-regulatory elements in promoter regions. Our results suggest that most PbCIPKs could play important roles in the abiotic stress responses. Some PbCIPKs, such as PbCIPK22, -19, -18, -15, -8, and -6 can serve as core regulators in response to salt and osmotic stresses based on co-expression networks of PbCIPKs. Some sets of genes that were involved in response to salt did not overlap with those in response to osmotic responses, suggesting the sub-functionalization of CIPK genes in stress responses. This study revealed some candidate genes that play roles in early responses to salt and osmotic stress for further characterization of abiotic stress responses medicated by CIPKs in pear.
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影响因子：
5.6
作者：
Kleist TJ;Spencley AL;Luan S
通讯作者：
Luan S
影响因子：
8.7
作者：
Du D;Rawat N;Deng Z;Gmitter FG Jr
通讯作者：
Gmitter FG Jr
影响因子：
2.9
作者：
Ma, Dong-Mei;Xu, Wei-Rong;Li, Hui-Wen;Jin, Feng-Xia;Guo, Ling-Na;Wang, Jing;Dai, Hong-Jun;Xu, Xing
通讯作者：
Xu, Xing
影响因子：
6.2
作者：
Hu, Da-Gang;Li, Ming;Hao, Yu-Jin
通讯作者：
Hao, Yu-Jin
影响因子：
7.4
作者：
Martinez-Atienza, Juliana;Jiang, Xingyu;Quintero, Francisco J.
通讯作者：
Quintero, Francisco J.