Comparative phylogenomics of the CBL-CIPK calcium-decoding network in the moss Physcomitrella, Arabidopsis, and other green lineages.
Comparative phylogenomics of the CBL-CIPK calcium-decoding network in the moss Physcomitrella, Arabidopsis, and other green lineages.
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DOI：
10.3389/fpls.2014.00187
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发表时间：
2014
影响因子：
5.6
通讯作者：
Luan S
中科院分区：
文献类型：
--
作者：
Kleist TJ;Spencley AL;Luan S
陆地植物已经进化出许多解剖学和分子适应陆地生长的能力。许多这些适应被认为是在它们的藻类祖先中存在的特征的细化。在拟南芥中，10种钙调神经磷酸酶b样蛋白（calcalineurin B-Like Protein, CBLs）作为钙传感器，调节26种钙调神经磷酸酶相互作用蛋白激酶（CIPKs）的活性。CBL-CIPK网络协调环境反应，帮助维持适当的离子平衡，特别是在非生物胁迫下。我们鉴定并分析了绿色谱系中CBL和CIPK的同源物，包括陆地植物最近的亲缘植物绿藻类的CBL和CIPK。系统基因组学证据表明，该网络从一个小模块扩展而来，可能是现代植物和藻类祖先中存在的单个CBL-CIPK对。在所有已鉴定的CIPK中，介导CBL-CIPK物理相互作用的NAF基序的极端保守性支持了绿藻和早期分化的陆地植物中CBL和CIPK同源物作为功能连接网络组件的解释。我们在小壶藓（一种模式苔藓）的基因组中发现了CBL和CIPK位点的完整补体。这些分析证明了最近苔藓全基因组重复的强大影响：CBL和CIPK位点出现在同源对中，其中一些似乎是假基因，具有高序列相似性。我们克隆了这些基因座的所有全长转录本，并进行酵母双杂交分析，以证明ccl - cipk相互作用，并确定网络中的特定连接。利用系统基因组学，我们鉴定了三种古老的CBLs类型，它们可以通过n端定位基序识别，以及一个“绿藻型”的CIPKs分支，其成员来自小壶属和拟南芥。
Land plants have evolved a host of anatomical and molecular adaptations for terrestrial growth. Many of these adaptations are believed to be elaborations of features that were present in their algal-like progenitors. In the model plant Arabidopsis, 10 Calcineurin B-Like proteins (CBLs) function as calcium sensors and modulate the activity of 26 CBL-Interacting Protein Kinases (CIPKs). The CBL-CIPK network coordinates environmental responses and helps maintain proper ion balances, especially during abiotic stress. We identified and analyzed CBL and CIPK homologs in green lineages, including CBLs and CIPKs from charophyte green algae, the closest living relatives of land plants. Phylogenomic evidence suggests that the network expanded from a small module, likely a single CBL-CIPK pair, present in the ancestor of modern plants and algae. Extreme conservation of the NAF motif, which mediates CBL-CIPK physical interactions, among all identified CIPKs supports the interpretation of CBL and CIPK homologs in green algae and early diverging land plants as functionally linked network components. We identified the full complement of CBL and CIPK loci in the genome of Physcomitrella, a model moss. These analyses demonstrate the strong effects of a recent moss whole genome duplication: CBL and CIPK loci appear in cognate pairs, some of which appear to be pseudogenes, with high sequence similarity. We cloned all full-length transcripts from these loci and performed yeast two-hybrid analyses to demonstrate CBL-CIPK interactions and identify specific connections within the network. Using phylogenomics, we have identified three ancient types of CBLs that are discernible by N-terminal localization motifs and a “green algal-type” clade of CIPKs with members from Physcomitrella and Arabidopsis.
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影响因子：
7.2
作者：
D'Angelo, Cecilia;Weinl, Stefan;Kudla, Joerg
通讯作者：
Kudla, Joerg
影响因子：
1.4
作者：
Eisen, JA;Wu, M
通讯作者：
Wu, M
影响因子：
3.4
作者：
FOGELSON, AL;ZUCKER, RS
通讯作者：
ZUCKER, RS
DOI：
10.1007/978-3-642-20829-4_12
发表时间：
2011-01-01
期刊：
CODING AND DECODING OF CALCIUM SIGNALS IN PLANTS
影响因子：
--
作者：
Batistic, Oliver;Kim, Kyung-Nam;Luan, Sheng
通讯作者：
Luan, Sheng
影响因子：
6.5
作者：
Anisimova, Maria;Gascuel, Olivier
通讯作者：
Gascuel, Olivier