The Distribution and Cooperation of Antioxidant (Iso)enzymes and Antioxidants in Different Subcellular Compartments in Maize Leaves during Water Stress
The Distribution and Cooperation of Antioxidant (Iso)enzymes and Antioxidants in Different Subcellular Compartments in Maize Leaves during Water Stress
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水分胁迫下玉米叶片不同亚细胞区室抗氧化（同）酶和抗氧化剂的分布与配合
DOI：
10.1007/s00344-010-9189-1
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发表时间：
2011-09
影响因子：
4.8
通讯作者：
Zhang Aying
中科院分区：
文献类型：
--
作者：
Zhang Aying
研究了聚乙二醇（PEG）诱导的轻度水分胁迫对玉米不同亚细胞室抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）]及其同工酶活性和抗氧化物[抗坏血酸（ASC）和谷胱甘肽（GSH）]含量的影响。对于每个亚细胞区室，几乎所有的同工酶的活动，解决了天然PAGE后4-12小时的暴露于水分胁迫和下降后，显示伴随着各自的总酶和抗氧化剂含量的活动的变化。对于每个亚细胞室，至少有一个异构体的检测抗氧化酶被解决，但不同种类的抗氧化同工酶在不同的亚细胞室有不同的响应水分胁迫。叶绿体中的Fe-SOD和线粒体中的Mn-SOD的相对贡献高于其它亚细胞区室。而质外体中的Mn-SOD和Fe-SOD活性在水分胁迫过程中呈下降趋势，而其它亚细胞区室中的Mn-SOD和Fe-SOD活性则相反。抗氧化酶活性的研究结果表明，在轻度水分胁迫下，各亚细胞区室中的抗氧化酶协同动员，同步响应，其活性变化趋势一致。这表明它们在原位清除活性氧（ROS）方面具有协同作用。此外，研究结果表明，线粒体和质外体，最积极的响应，可能是目标，以改善植物的性能在轻度水分胁迫。
The effects of mild water stress induced by polyethylene glycol (PEG) on the activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR)] and their isoenzymes and the antioxidant content [ascorbate (ASC) and glutathione (GSH)] of different subcellular compartments were investigated in maize. For each subcellular compartment, the activities of almost all isoenzymes resolved on native PAGE increased after 4–12 h of exposure to water stress and declined after that, showing concomitant changes with the activities of their respective total enzymes and the antioxidant content. For each subcellular compartment, at least one isoform for the detected antioxidant enzymes was resolved, but different kinds of antioxidant isoenzymes in different subcellular compartments had different responses to water stress. The relative contribution of Fe–SOD in chloroplasts and Mn–SOD in mitochondria was higher than that in other subcellular compartments. However, in apoplasts the activities of Mn–SOD and Fe–SOD declined during the process of water stress, in contrast to those located in other subcellular compartments. The results from the activities of antioxidant (iso)enzymes demonstrated that all antioxidant enzymes in all subcellular compartments were mobilized in cooperation and responded synchronously under mild water stress, with the same trend of changes in their activity. This indicated their orchestrated effects in scavenging reactive oxygen species (ROS) in situ. Additionally, the results suggested that mitochondria and apoplasts, responding most actively, might be targets for improving plant performance under mild water stress.
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影响因子：
6.9
作者：
Jubany-Marí T;Munné-Bosch S;López-Carbonell M;Alegre L
通讯作者：
Alegre L
土壤渐进干燥过程中冬小麦化学信号和耐旱性的土壤-水阈值范围是由活性氧稳态介导的
影响因子：
4.8
作者：
Xiong, You-Cai;Xu, Bing-Cheng;Li, Feng-Min;Wang, Zhen-Yu
通讯作者：
Wang, Zhen-Yu
DOI：
10.1074/jbc.274.47.33274
发表时间：
1999-11
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
T. Yamahara;Tadahiko Shiono;Takanori Suzuki;Katuyuki Tanaka;S. Takio;Kiichi Sato;S. Yamazaki;T. Satoh
通讯作者：
T. Yamahara;Tadahiko Shiono;Takanori Suzuki;Katuyuki Tanaka;S. Takio;Kiichi Sato;S. Yamazaki;T. Satoh
影响因子：
6.4
作者：
Locato, Vittoria;de Pinto, Maria Concetta;De Gara, Laura
通讯作者：
De Gara, Laura
影响因子：
4.2
作者：
Contour-Ansel, Dominique;Torres-Franklin, Maria Lucia;D'Arcy-Lameta, Agnes
通讯作者：
D'Arcy-Lameta, Agnes