Anopheles funestus Populations across Africa Are Broadly Susceptible to Neonicotinoids but with Signals of Possible Cross-Resistance from the GSTe2 Gene.
Anopheles funestus Populations across Africa Are Broadly Susceptible to Neonicotinoids but with Signals of Possible Cross-Resistance from the GSTe2 Gene.
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DOI：
10.3390/tropicalmed8050244
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发表时间：
2023-04-24
影响因子：
2.9
通讯作者：
Wondji CS
中科院分区：
文献类型：
--
作者：
Assatse T;Tchouakui M;Mugenzi L;Menze B;Nguiffo-Nguete D;Tchapga W;Kekeunou S;Wondji CS
评估疟疾病媒对世卫组织推荐的新产品的敏感性是大规模部署之前的一个关键步骤。我们绘制了非洲各地的按蚊对烟碱类杀虫剂的敏感性分布图，并以丙酮+ MERO为溶剂建立了啶虫脒和吡虫啉的诊断剂量。室内休息安。2021年，在喀麦隆、马拉维、加纳和乌干达采集了funestus。使用CDC瓶测定法和田间捕获的成虫的后代对噻虫胺、吡虫啉和啶虫脒的敏感性进行了评价。对L119 F-GSTe 2标记物进行基因分型，以评估噻虫胺与该DDT/拟除虫菊酯抗性标记物之间的潜在交叉抗性。蚊子对丙酮+ MERO中稀释的三种类烟碱敏感，而单独使用乙醇或丙酮时死亡率较低。用丙酮+ MERO分别确定6 µg/mL和4 µg/mL的剂量为吡虫啉和啶虫脒的诊断浓度。预暴露于增效剂显着恢复的敏感性噻虫胺。L119 F-GSTe 2突变与噻虫胺抗性呈正相关，纯合子抗性蚊子比杂合子或易感蚊子更能存活。这项研究表明，安。非洲各地的funestus种群对类烟碱敏感，因此，可以使用IRS有效地使用这类杀虫剂来控制这一物种。然而，潜在的交叉耐药性赋予GSTe 2要求定期监测耐药领域。
Evaluating the susceptibility of malaria vectors to the new WHO-recommended products is a key step before large-scale deployment. We mapped the susceptibility profile of Anopheles funestus to neonicotinoids across Africa and established the diagnostic doses of acetamiprid and imidacloprid with acetone + MERO as solvent. Indoor resting An. funestus were collected in 2021 in Cameroon, Malawi, Ghana and Uganda. Susceptibility to clothianidin, imidacloprid and acetamiprid was evaluated using CDC bottle assays and offsprings of the field-caught adults. The L119F-GSTe2 marker was genotyped to assess the potential cross-resistance between clothianidin and this DDT/pyrethroid-resistant marker. Mosquitoes were susceptible to the three neonicotinoids diluted in acetone + MERO, whereas low mortality was noticed with ethanol or acetone alone. The doses of 6 µg/mL and 4 µg/mL were established as diagnostic concentrations of imidacloprid and acetamiprid, respectively, with acetone + MERO. Pre-exposure to synergists significantly restored the susceptibility to clothianidin. A positive correlation was observed between L119F-GSTe2 mutation and clothianidin resistance with the homozygote resistant mosquitoes being more able to survive than heterozygote or susceptible. This study revealed that An. funestus populations across Africa are susceptible to neonicotinoids, and as such, this insecticide class could be effectively implemented to control this species using IRS. However, potential cross-resistance conferred by GSTe2 calls for regular resistance monitoring in the field.
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