Side population cells from human melanoma tumors reveal diverse mechanisms for chemoresistance.
Side population cells from human melanoma tumors reveal diverse mechanisms for chemoresistance.
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DOI：
10.1038/jid.2012.161
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发表时间：
2012-10
影响因子：
6.5
通讯作者：
Fujita, Mayumi
中科院分区：
文献类型：
--
作者：
Luo, Yuchun;Ellis, Lixia Z.;Dallaglio, Katiuscia;Takeda, Moe;Robinson, William A.;Robinson, Steven E.;Liu, Weimin;Lewis, Karl D.;McCarter, Martin D.;Gonzalez, Rene;Norris, David A.;Roop, Dennis R.;Spritz, Richard A.;Ahn, Natalie G.;Fujita, Mayumi
Side population (SP) is identified as cells capable of excluding the fluorescent Hoechst dye and anticancer drugs, and represents hematopoietic stem cells and chemoresistant cells from several solid tumors. In this study, we confirmed the presence of SP cells in tumors from melanoma patients. Melanoma SP cells overexpressed ATP-binding-cassette (ABC) transporters, ABCB1 and ABCB5. We generated a direct in vivo xenograft model, and demonstrated that SP cells were resistant to paclitaxel, a substrate of ABCB1, both in vitro and in vivo. However, melanoma SP cells were also resistant to temozolomide, which is not a substrate for ABC transporters, through IL-8 upregulation. In addition, gene profiling studies identified three signaling pathways (NF- κB, α6-β4-integrin and IL-1) as differentially upregulated in melanoma SP cells, and there was a significant increase of PCDHB11 and decrease of FUK and TBX2 in these cells. Therefore, we provide evidence that SP is an enriched source of chemoresistant cells in human melanomas, and suggest that the selected genes and signaling pathways of SP cells may be a potential target for effective melanoma therapies. To our knowledge, this is previously unreported study to isolate SP cells from melanoma patients and to investigate the gene expression profiling of these cells.
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影响因子：
2.5
作者：
Dean, Michael
通讯作者：
Dean, Michael
影响因子：
11.2
作者：
Frank, NY;Margaryan, A;Frank, MH
通讯作者：
Frank, MH
影响因子：
11.2
作者：
Daniel VC;Marchionni L;Hierman JS;Rhodes JT;Devereux WL;Rudin CM;Yung R;Parmigiani G;Dorsch M;Peacock CD;Watkins DN
通讯作者：
Watkins DN
影响因子：
8.4
作者：
Chelouche-Lev, D;Miller, CP;Price, JE
通讯作者：
Price, JE
影响因子：
2.6
作者：
Boeckmann, Lars;Schirmer, Markus;Emmert, Steffen
通讯作者：
Emmert, Steffen