Adenosine Triphosphate and Adenosine Increase the Pulmonary Blood Flow to Postnatal Levels in Fetal Lambs
Adenosine Triphosphate and Adenosine Increase the Pulmonary Blood Flow to Postnatal Levels in Fetal Lambs
复制标题
三磷酸腺苷和腺苷可将胎羔的肺血流量增加至产后水平
作者：
G. Konduri;A. Theodorou;A. Mukhopadhyay;D. R. Deshmukh
摘要：我们研究了嘌呤核苷酸可能介导胎羊羔球茎处肺血管舒张的假设。我们研究了放置血管内导管、左肺动脉周围的血流传感器和动脉导管周围的充气式血管闭塞器后3天的9只胎儿羔羊。研究了动脉导管短暂闭塞时左肺压力-血流关系作为肺血管阻力的指标。研究了右心房线灌注0.01 ~ 2.50 μmol/kg/min剂量的腺苷、ATP或生理盐水（对照）对肺血管的影响，并测定了肺动脉和左心房样本血中腺苷和ATP的水平。我们还探讨了腺苷和ATP对肺血管的作用机制。0.08 ~ 2.5 μmol/kg/min剂量下，腺苷和ATP显著降低肺血管阻力，增加肺血流量。在1.2和2.5 μmol/kg/min的腺苷和ATP剂量下，肺血流量增加到出生后羔羊的水平。胎儿血液中腺苷和三磷酸腺苷的基线水平分别为出生后羔羊的8%和70%。在1.2 μmol/ kg/rain和23 μmol/ kg/rain的输注速率下，ATP浓度升高至出生后水平，腺苷浓度升高至出生后水平的20%。8-苯基茶碱能减弱腺苷和ATP引起的肺血管舒张，吲哚美辛不能减弱。我们得出结论，腺苷和三磷酸腺苷是肺血管扩张剂，在将其血药浓度增加到≤产后水平的剂量下，可将胎儿肺流量增加到产后水平。腺苷和ATP的作用是通过刺激P1和P2 pnrinergk受体介导的，与前列环素的合成无关。嘌呤核苷酸可能是围产期羔羊肺血管舒张的重要介质。
ABSTRACT: We investigated the hypothesis that purine nucleotides may mediate the pulmonary vasodilation that occurs at bulb in fetal lambs. We studied nine fetal lambs 3 d after placement of intravascular catheters, a flow transducer around the left pulmonary artery, and an inflatable vascular occluder around the ductus arteriosus. The pressure-flow relationship of left lung during a brief occlusion of the ductus arteriosus was studied as an index of pulmonary vascular resistance. We investigated the pulmonary vascular effects of adenosine, ATP, or saline (control) in doses of 0.01–2.50 μmol/kg/min infused into the right atrial line, and measured blood adenosine and ATP levels in samples from the pulmonary artery and left atrium. We also investigated the mechanism of pulmonary vascular effects of adenosine and ATP. Adenosine and ATP caused significant decreases in pulmonary vascular resistance and increases in pulmonary blood flow in doses of 0.08–2.5 μmol/kg/min. The pulmonary blood flow increased to levels seen in postnatal lambs at doses of 1.2 and 2.5 μmol/kg/min of adenosine and ATP. The baseline blood adenosine and ATP levels in fetus were 8 and 70% of levels in postnatal lambs. ATP concentrations increased to postnatal levels and adenosine levels increased to 20% of postnatal levels at infusion rates of 1.2 and 23 μmol/ kg/rain. The pulmonary vasodilation caused by adenosine and ATP was attenuated by 8-phenyltheophylline and ci-bacron blue, respectively, but not by indomethacin. We conclude that adenosine and ATP are pulmonary vasodilators and increase the fetal pulmonary flow to postnatal levels in doses that increase their blood concentrations to ≤ postnatal levels. The effects of adenosine and ATP are mediated by stimulation of P1 and P2 pnrinergk receptors and are independent of prostacyclin synthesis. Purine nucleotides may be important mediators of pulmonary vasodilation that occurs in the perinatal Iamb.
影响因子：
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作者：
MORIN, FC;EGAN, EA;LUNDGREN, CEG
通讯作者：
LUNDGREN, CEG
影响因子：
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作者：
ABMAN, SH;ACCURSO, FJ
通讯作者：
ACCURSO, FJ
腺苷介导的冠状动脉平滑肌中环AMP依赖性蛋白激酶的松弛和激活。
DOI：
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发表时间：
1984
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Silver,PJ;Walus,K;DiSalvo,J
通讯作者：
DiSalvo,J