Streptococcus suis serotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Streptococcus suis serotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
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DOI：
10.1371/journal.pone.0249682
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Nuanualsuwan S
中科院分区：
文献类型：
--
作者：
Chaiden C;Jaresitthikunchai J;Kerdsin A;Meekhanon N;Roytrakul S;Nuanualsuwan S
猪链球菌，特别是猪链球菌血清2型（SS2），是世界范围内引起人类脑膜炎的重要人畜共患病原体。虽然病原和致病性血清型的正确分类对临床诊断是有益的，但目前的血清分型方法导致无法区分血清型的交叉反应是显着的局限性。本研究采用基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF-MS）对猪链球菌提取的多肽进行分析，以改进猪链球菌血清型的分类。利用猪链球菌32株参考菌株的全细胞肽，建立猪链球菌的肽质量指纹图谱（PMFs）。从泰国临床病例中分离的32株人类猪链球菌用于验证这种替代血清分型方法，并与多重(m)PCR方法进行直接比较。所有参考菌株，代表32种猪链球菌血清型，表现出各自的PMFs模式，从而允许彼此分化。高致病性的SS2和SS14分别与血清学上密切相关的SS1/2和SS1有明显的区别。所建立的MALDI-TOF-MS血清分型方法对来自PMFs数据库的68.8%（22/32）的相同血清型分离株进行了正确的分型；临床分离株的效度为62.5%（20/32）。MALDI-TOF-MS与mPCR血清分型的一致性为中等，Kappa评分为0.522，考虑到mPCR的血清分型正确率高达75%。本研究表明，基于maldi - tof - ms方法的PMFs可以成功地将以前无法区分的高致病性SS2和SS14分别与SS1/2和SS1区分开来。此外，该血清分型方法可区分致病性SS6，因此是快速可靠地对临床致病性猪链球菌分离物进行血清分型的另一种选择方法。
Streptococcus suis, particularly S. suis serotype 2 (SS2), is an important zoonotic pathogen causing meningitis in humans worldwide. Although the proper classification of the causative and pathogenic serotype is salutary for the clinical diagnosis, cross-reactions leading to the indistinguishability of serotypes by the current serotyping methods are significant limitations. In the present study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of extracted peptides was developed to improve the classification of serotype of S. suis. The peptide mass fingerprint (PMFs) database of S. suis was generated from the whole-cell peptides of 32 reference strains of S. suis isolates obtained from pigs. Thirty-two human S. suis isolates from clinical cases in Thailand were used to validate this alternative serotyping method in direct comparison to the multiplex (m)PCR approach. All reference strains, representing 32 serotypes of S. suis, exhibited their individual PMFs patterns, thus allowing differentiation from one another. Highly pathogenic SS2 and SS14 were clearly differentiated from the otherwise serologically closely related SS1/2 and SS1, respectively. The developed MALDI-TOF-MS serotyping method correctly classified the serotype in 68.8% (22/32) of the same serotype isolates generated from the PMFs database; while the validity for the clinical human isolates was 62.5% (20/32). The agreement between the MALDI-TOF-MS and mPCR serotyping was moderate with a Kappa score of 0.522, considering that mPCR could correctly serotype up to 75%. The present study demonstrated that PMFs from the developed MALDI-TOF-MS-based method could successfully discriminate the previously indistinguishable highly pathogenic SS2 and SS14 from SS1/2 and SS1, respectively. Moreover, this serotyping method distinguished pathogenic SS6, and so is an alternative approach of choice to rapidly and reliably serotype clinically pathogenic S. suis isolates.
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MALDI-TOF MS作为诊断从猪的临床标本中回收的链球菌物种的诊断工具的有用性。
影响因子：
3.7
作者：
Pérez-Sancho M;Vela AI;García-Seco T;González S;Domínguez L;Fernández-Garayzábal JF
通讯作者：
Fernández-Garayzábal JF
影响因子：
3.3
作者：
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影响因子：
3.3
作者：
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通讯作者：
Osawa, Ro
影响因子：
9.4
作者：
Alatoom, Adnan A.;Cunningham, Scott A.;Patel, Robin
通讯作者：
Patel, Robin
影响因子：
11.8
作者：
Seng, Piseth;Drancourt, Michel;Raoult, Didier
通讯作者：
Raoult, Didier