Age dependency of vasopressin pulmonary vasodilatory effect in rats.
Age dependency of vasopressin pulmonary vasodilatory effect in rats.
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DOI：
10.1038/pr.2013.221
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发表时间：
2014-02
影响因子：
3.6
通讯作者：
Belik, Jaques
中科院分区：
文献类型：
--
作者：
Enomoto, Masahiro;Pan, Jingyi;Shifrin, Yulia;Belik, Jaques
加压素是一种全身性血管收缩药。它的肺血管扩张作用存在争议，关于它在新生儿肺动脉高压中的应用数据有限。假设加压素诱导的肺血管扩张是发育调节的，我们在新生和成年大鼠身上评估了它的肺和全身动脉反应。血管安装在钢丝肌图仪上，测量血管加压素引起的血管运动张力的变化。Western blotting分析血管和年龄相关血管加压素V1a和V2受体表达的差异。在这两个年龄段，血管加压素引起肠系膜动脉张力的剂量依赖性增加，但在成人血管中增加幅度更大(P<；0.01)。在较低浓度下，加压素在成人血管中引起肺血管扩张，在新生儿动脉中引起血管收缩。布洛芬可抑制成人加压素引起的肺血管扩张，提示该反应是前列腺素介导的。成人肺组织V1a受体蛋白表达高于新生动脉(P<；0.01)。成年血管V1a主要在肺动脉表达，V2仅在肠系膜动脉表达。新生大鼠没有血管加压素诱导的肺血管扩张，这可能是由于出生早期组织V1a表达较低所致。这些动物数据对加压素在新生儿肺动脉高压中的治疗作用提出了挑战。
Vasopressin is a systemic vasoconstrictor. Its pulmonary vasodilatory effect is controversial and limited data are available on its use in neonates with pulmonary hypertension. Hypothesizing that the vasopressin-induced pulmonary vasodilation is developmentally regulated, we evaluated its pulmonary and systemic arterial response in newborn and adult rats. Vessels were mounted on a wire myograph and the vasopressin-induced changes in vasomotor tone measured. The vessel- and age-dependent differences in vasopressin V1a and V2 receptors expression were evaluated by Western blotting. Vasopressin induced a dose-dependent increase in mesenteric arterial tone at both ages, but of greater magnitude in adult vessels (P<0.01). At lower concentrations, vasopressin induced pulmonary vasodilation in adult vessels and vasoconstriction in newborn arteries. The adult vasopressin-induced pulmonary vasodilation was inhibited by ibuprofen suggesting that the response is prostaglandin mediated. Pulmonary tissue V1a receptor protein expression was higher in adult, when compared with newborn arteries (P<0.01). The adult vessels V1a expression predominated in the pulmonary arteries and V2 was only detected in mesenteric arteries. The vasopressin-induced pulmonary vasodilation is absent in newborn rats likely due to the lower tissue V1a expression early in life. These animal data challenge the therapeutic use of vasopressin in neonatal pulmonary hypertension.
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DOI：
10.1152/ajpheart.1994.267.6.h2413
发表时间：
1994-12-01
影响因子：
4.8
作者：
EICHINGER, MR;WALKER, BR
通讯作者：
WALKER, BR
影响因子：
2
作者：
Shivananda, Sandesh;Ahliwahlia, Lucia;McNamara, Patrick
通讯作者：
McNamara, Patrick
影响因子：
3.2
作者：
Medina, P;Segarra, G;Lluch, S
通讯作者：
Lluch, S
DOI：
10.1186/cc9264
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Price LC;Wort SJ;Finney SJ;Marino PS;Brett SJ
通讯作者：
Brett SJ
影响因子：
3.7
作者：
Rieg AD;Rossaint R;Uhlig S;Martin C
通讯作者：
Martin C