Targeting the skin for genetic immunization.
Targeting the skin for genetic immunization.
复制标题
针对皮肤进行基因免疫。
DOI：
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复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Louis D. Falo
中科院分区：
文献类型：
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作者：
Louis D. Falo
基因治疗最新进展的最有前途的应用之一是开发基于抗原编码DNA递送的免疫策略。基于DNA的疫苗，也被称为基因疫苗或多核苷酸疫苗，在改进现有免疫策略方面提供了相当大的前景，皮肤作为基因疫苗的目标组织具有独特的潜力。基因导入的抗原在富含专业抗原提呈细胞和辅助细胞的皮肤微环境中的表达，能够产生免疫刺激细胞因子，有可能克服疫苗和免疫治疗的历史局限性。尽管基因免疫的确切分子机制尚不清楚，但可以构建一个通用的工作模型，通过这些事件，将抗原编码的质粒引入皮肤，从而启动免疫反应。朗格汉斯细胞可以在体内转基因的发现，增加了这些迁移的专业抗原呈递细胞可以在体内进行基因工程的令人兴奋的可能性。通过设计策略将编码抗原的基因和编码免疫调节分子的基因共同递送到同一抗原提呈细胞，有可能在宿主中诱导或抑制抗原特异性免疫反应。尽管皮肤DNA免疫生物学的许多方面尚不清楚，但皮肤似乎为将基因治疗的进展应用于疫苗接种和免疫反应的基因工程提供了独特的潜力。
One of the most promising applications of recent advances in gene therapy is the development of immunization strategies based on the delivery of antigen-encoding DNA. DNA-based vaccination, also referred to as genetic vaccination or polynucleotide vaccination, offers considerable promise for improvement over existing immunization strategies, and the skin offers unique potential as a target tissue for genetic vaccines. The expression of genetically introduced antigens in a cutaneous microenvironment rich in both professional antigen-presenting cells and accessory cells, which are capable of producing immunostimulatory cytokines, has the potential to overcome the historical limitations of vaccinology and immunotherapy. Though the precise molecular mechanisms of genetic immunization remain unclear, a general working model of the events through which antigen-encoding plasmids introduced into the skin initiate an immune response can be constructed. The finding that Langerhans cells can be transfected in vivo raises the exciting possibility that these migrating professional antigen-presenting cells can be genetically engineered in vivo. By designing strategies to codeliver genes encoding antigens with genes encoding immunoregulatory molecules to the same antigen-presenting cell, it may be possible to either induce or suppress antigen-specific immune responses in the host. Though many aspects of the biology of cutaneous DNA immunization remain unknown, the skin appears to offer unique potential for the application of advances in gene therapy to vaccination and genetic engineering of the immune response.
DOI：
10.1073/pnas.90.24.11478
发表时间：
1993-12-15
影响因子：
11.1
作者：
FYNAN, EF;WEBSTER, RG;ROBINSON, HL
通讯作者：
ROBINSON, HL
基因枪和肌内 DNA 免疫对靶位点组织的不同依赖性。
DOI：
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发表时间：
1997
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Torres,CA;Iwasaki,A;Barber,BH;Robinson,HL
通讯作者：
Robinson,HL
DOI：
10.1073/pnas.93.10.5141
发表时间：
1996-05-14
影响因子：
11.1
作者：
Raz, E;Tighe, H;Carson, DA
通讯作者：
Carson, DA