Association of Neisseria gonorrhoeae genogroups and specific PBP2/MtrR/PorB mutation patterns with susceptibility to penicillin in a susceptible gonococcal population
Association of Neisseria gonorrhoeae genogroups and specific PBP2/MtrR/PorB mutation patterns with susceptibility to penicillin in a susceptible gonococcal population
复制标题
淋病奈瑟菌基因组和特定 PBP2/MtrR/PorB 突变模式与易感淋球菌人群对青霉素敏感性的关联
DOI：
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发表时间：
2018
影响因子：
5.2
通讯作者：
J. Dillon
中科院分区：
文献类型：
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作者：
S. D. Thakur;P. Levett;G. Horsman;J. Dillon
目标
旨在确定对青霉素具有不同敏感性的淋病奈瑟菌分离株的抗菌敏感性是否与基因组 (GG) 以及 PBP2 (penA)、多重转移耐药阻遏物 (MtrR; mtrR) 和孔蛋白 B (PorB; porB) 的组合突变模式相关。
方法
采用琼脂稀释法和CLSI判读标准测定了146株临床分离的淋病奈瑟菌对青霉素的敏感性。将分离株中 penA、mtrR 和 porB 的 DNA 序列与 WT 序列进行比较，并确定突变模式。通过淋病奈瑟菌多抗原序列分型 (NG-MAST) 对分离株进行分型，并将 ST 分为特定的 GG。
结果
测试的分离株在 PBP2 中携带 9 种突变模式，在 MtrR 和 PorB 中携带 12 种突变模式。在 146 个分离株中，121 个（82.9%）被分为 13 个不同的 GG。青霉素 MIC 为 0.03-0.06mg/L 的分离株与 GG25 (P<0.05) 和 PBP2/MtrR/PorB 突变模式 I/WT/WT 显着相关 (P<0.01)。青霉素 MIC 为 1.0mg/L 的分离株与以下因素相关 (P<0.05)： (i) GG3655 和突变模式 XXII/A-；G45D/G120K；A121N； (ii) GG921和突变模式IX/G45D/G120D；A121N； (iii) GG1109 和突变模式 IX/G45D/WT。百分之六十 (9/15) 的青霉素耐药菌株 (MIC ≥2mg/L) 为 GG3654 (P<0.0001)，并携带突变模式 IX/G45D/G120K;A121D 或 IX/G45D/G120D;A121D (P<0.05)。
结论
PBP2/MtrR/PorB 中的特定突变模式与特定的 GG 和青霉素敏感性相关。这种对菌株和耐药性模式进行分型的方法非常适合预测抗菌药物耐药性，并且应用于无法获得淋球菌培养物但可以从临床标本中获得 DNA 的情况。
Objectives
To ascertain whether the antimicrobial susceptibility of Neisseria gonorrhoeae isolates with differing susceptibilities to penicillin is associated with genogroups (GGs) and combined mutation patterns in PBP2 (penA), the multiple transfer resistance repressor (MtrR; mtrR) and porin B (PorB; porB).
Methods
The susceptibility of 146 clinical N. gonorrhoeae isolates to penicillin was determined using the agar dilution method and the interpretation criteria of CLSI. The DNA sequences of penA, mtrR and porB in isolates were compared with WT sequences and mutation patterns were determined. Isolates were typed by N. gonorrhoeae multi-antigen sequence typing (NG-MAST) and STs were grouped into specific GGs.
Results
The isolates tested carried 9 mutation patterns in PBP2 and 12 mutation patterns in each of MtrR and PorB. Of the 146 isolates, 121 (82.9%) were grouped into 13 different GGs. Isolates with penicillin MICs of 0.03-0.06 mg/L were significantly associated with GG25 (P < 0.05) and PBP2/MtrR/PorB mutation pattern I/WT/WT (P < 0.01). Isolates with a penicillin MIC of 1.0 mg/L were associated (P < 0.05) with: (i) GG3655 and mutation pattern XXII/A-;G45D/G120K;A121N; (ii) GG921 and mutation pattern IX/G45D/G120D;A121N; and (iii) GG1109 and mutation pattern IX/G45D/WT. Sixty percent (9/15) of penicillin-resistant isolates (MIC ≥2 mg/L) were GG3654 (P < 0.0001) and carried mutation pattern IX/G45D/G120K;A121D or IX/G45D/G120D;A121D (P < 0.05).
Conclusions
Specific mutation patterns in PBP2/MtrR/PorB were associated with specific GGs and penicillin susceptibility. This approach of typing strains and resistance patterns is ideal for predicting antimicrobial resistance and should be used in instances in which gonococcal culture is not available but DNA can be obtained from clinical specimens.
影响因子：
5.2
作者：
Zarantonelli, L;Borthagaray, G;Shafer, WM
通讯作者：
Shafer, WM