Changes in the extracellular microenvironment and osteogenic responses of mesenchymal stem/stromal cells induced by in vitro direct electrical stimulation.
Changes in the extracellular microenvironment and osteogenic responses of mesenchymal stem/stromal cells induced by in vitro direct electrical stimulation.
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DOI：
10.1177/2041731420974147
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发表时间：
2021-01
影响因子：
8.2
通讯作者：
Cartmell SH
中科院分区：
文献类型：
--
作者：
Srirussamee K;Xue R;Mobini S;Cassidy NJ;Cartmell SH
电刺激（ES）有可能成为临床治疗骨损伤的有效工具。然而，与ES相关的治疗机制仍在讨论中。本研究旨在通过表征ES过程中细胞外环境的物理和化学变化，并将其与间充质干细胞/基质细胞（MSC）的反应相关联，来研究其初始作用机制。使用计算建模来估计相对于阴极的电位和穿过细胞单层的电流密度。我们发现磷酸化ERK 1/2，c-FOS，c-JUN和SPP 1 mRNA的表达，以及MSC在不同时间点的代谢活性增加。此外，从电刺激培养基（ES培养基）中测得平均2.5 μM的H2 O2和34 μg/L的溶解Pt，这也与SPP 1 mRNA表达和细胞代谢活性的增加相对应。丙酮酸钠作为抗氧化剂加入到ES培养基中并没有改变SPP 1 mRNA的表达，但消除了ES培养基处理诱导的细胞代谢活动的增加。这些结果表明，H2 O2影响细胞的代谢活动，而SPP 1 mRNA的表达受到其他感应副产物的调节。这项研究揭示了不同的电刺激方案如何改变细胞再生反应和感应副产物的作用，这可能被用作指导和控制细胞行为的物理工具。
Electrical stimulation (ES) has potential to be an effective tool for bone injury treatment in clinics. However, the therapeutic mechanism associated with ES is still being discussed. This study aims to investigate the initial mechanism of action by characterising the physical and chemical changes in the extracellular environment during ES and correlate them with the responses of mesenchymal stem/stromal cells (MSCs). Computational modelling was used to estimate the electrical potentials relative to the cathode and the current density across the cell monolayer. We showed expression of phosphorylated ERK1/2, c-FOS, c-JUN, and SPP1 mRNAs, as well as the increased metabolic activities of MSCs at different time points. Moreover, the average of 2.5 μM of H2O2 and 34 μg/L of dissolved Pt were measured from the electrically stimulated media (ES media), which also corresponded with the increases in SPP1 mRNA expression and cell metabolic activities. The addition of sodium pyruvate to the ES media as an antioxidant did not alter the SPP1 mRNA expression, but eliminated an increase in cell metabolic activities induced by ES media treatment. These findings suggest that H2O2 was influencing cell metabolic activity, whereas SPP1 mRNA expression was regulated by other faradic by-products. This study reveals how different electrical stimulation regime alters cellular regenerative responses and the roles of faradic by-products, that might be used as a physical tool to guide and control cell behaviour.
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影响因子：
2.5
作者：
Banks, T. A.;Luckman, P. S. B.;Cooper-White, J. J.
通讯作者：
Cooper-White, J. J.
影响因子：
3.3
作者：
Chellaiah, MA;Kizer, N;Hruska, KA
通讯作者：
Hruska, KA
影响因子：
64.8
作者：
BASSETT, CA;PAWLUK, RJ;BECKER, RO
通讯作者：
BECKER, RO
DOI：
10.1152/ajpendo.00450.2013
发表时间：
2014-04-01
影响因子：
5.1
作者：
Bustamante, Mario;Fernandez-Verdejo, Rodrigo;Buvinic, Sonja
通讯作者：
Buvinic, Sonja
DOI：
10.1152/ajpheart.2001.280.5.h2357
发表时间：
2001-05-01
影响因子：
4.8
作者：
Cho, M;Hunt, TK;Hussain, MZ
通讯作者：
Hussain, MZ