Parathyroid hormone inhibits plasma membrane Pi transport without changing endocytic activity in opossum kidney cells.
Parathyroid hormone inhibits plasma membrane Pi transport without changing endocytic activity in opossum kidney cells.
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甲状旁腺激素抑制质膜 Pi 转运而不改变负鼠肾细胞的内吞活性。
DOI：
10.1016/0167-4889(95)00008-g
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Kempson,SA
中科院分区：
文献类型：
--
作者：
Paraiso,MS;McAteer,JA;Kempson,SA
Parathyroid hormone (PTH) inhibits Na+-dependent Pi uptake in renal epithelial cells from opossum kidney (OK). This requires an intact endocytic pathway, suggesting that one action of PTH may be to promote endocytic removal of Na+/Pi cotransporters from the cell membrane. The present study tested if PTH, at a dose that inhibited membrane Pi transport, also produced an increase in endocytic activity. Pi transport was measured in isolated plasma membrane vesicles. Endocytosis was measured by allowing cells to take up horseradish peroxidase (HRP) followed by assay of triton-sensitive (latent) HRP activity in subcellular fractions isolated by density gradient centrifugation. Incubation of OK cells with 10−7M PTH for 3 h decreased Na+/Pi cotransport by membrane vesicles to 328 ± 54 pmol/mg/min compared to 448 ± 67 pmol/mg/min (mean ± S.E., P < 0.03) in controls. Latent HRP content of endosomal fractions was dependent on the time and temperature used to load cells with HRP and on the concentration of HRP. However, incubation of OK cells with 10−7M PTH for either 1 or 3 h produced no change in latent HRP activity. Thus the action of PTH on the Na+/Pi cotransporter in the plasma membrane of OK cells does not require a change in the rate of endocytosis.
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作者：
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DOI：
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发表时间：
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期刊：
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DOI：
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发表时间：
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期刊：
Biochimica et biophysica acta
影响因子：
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