D1‐D2 complex of the photosystem II reaction center from spinach Isolation and partial characterization
D1‐D2 complex of the photosystem II reaction center from spinach Isolation and partial characterization
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菠菜分离和部分表征中光系统 II 反应中心的 D1-D2 复合物
DOI：
10.1016/0014-5793(90)81098-9
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
K. Satoh
中科院分区：
文献类型：
--
作者：
Xiao;K. Fushimi;K. Satoh
由D1和D2蛋白组成的色素-蛋白复合物，在光系统II的两个较低分子质量组分中，即cytochromeb - 559和psbigene产物中被耗尽，已经通过辛基- β - D - glucopyranoside处理纯化的菠菜光系统II反应中心复合物[(1987)Proc. Natl]。学会科学。【美国文献84,109‐112】，然后使用凝胶渗透柱（TSK G3000 SW）进行高效液相色谱分离。在稳态光照下，在二硫代盐和紫紫甲基存在下，分离的配合物在内在叶绿素的光还原中具有光化学活性，并且表现出与未处理反应中心相似的色素化学统计，这表明D1‐D2配合物在光系统II中提供了初级光化学位点，以及反应中心色素的主要结合位点。
A pigment‐protein complex consisting of D1 and D2 proteins, but depleted in the two lower molecular mass components of photosystem II, i.e. cytochromeb‐559 andpsbIgene product, has been isolated by octyl‐β‐D‐glucopyranoside treatment of the purified photosystem II reaction center complex from spinach [(1987) Proc. Natl. Acad. Sci. USA 84, 109‐112], followed by separation by high performance liquid chromatography using a gel‐permeation column (TSK G3000 SW). The isolated complex is photochemically active in the photoreduction of intrinsic pheophytinaunder steady‐state illumination, in the presence of dithionite and methyl viologen, and exhibits pigment stoichiometries similar to those in the untreated reaction center, indicating that the D1‐D2 complex provides the site of primary photochemistry in photosystem II, as well as the principal binding sites of pigments in the reaction center.
47 kDa 蛋白和 D1-D2-细胞色素 b-559 复合物的分离和表征。
DOI：
10.1016/s0005-2728(89)80164-1
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Ghanotakis,DF;dePaula,JC;Demetriou,DM;Bowlby,NR;Petersen,J;Babcock,GT;Yocum,CF
通讯作者：
Yocum,CF