Dynamic Changes in the Splenic Transcriptome of Chickens during the Early Infection and Progress of Marek's Disease.
Dynamic Changes in the Splenic Transcriptome of Chickens during the Early Infection and Progress of Marek's Disease.
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马立克氏病早期感染及进展过程中鸡脾脏转录组的动态变化
DOI：
10.1038/s41598-017-11304-y
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发表时间：
2017-09-14
影响因子：
4.6
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Dang L;Teng M;Li HW;Li HZ;Ma SM;Zhao P;Li XJ;Deng RG;Zhang GP;Luo J
鸡α疱疹病毒2型(GaHV2)是一种致癌性禽疱疹病毒，可引起马立克氏病(MD)和快速发病的T细胞淋巴瘤。为了揭示MD发病和肿瘤发生中的分子事件，利用RNA-seq技术检测了GaHV2感染雏鸡在感染早期和致病阶段的动态脾转录组。基于显著差异表达基因(Degs)的基因本体论分析、KEGG途径分析和蛋白质-蛋白质相互作用网络分析表明，GaHV2感染过程中发生的分子事件与疾病进程密切相关。在“康奈尔模型”对MD的描述中，先天免疫反应和炎症反应是在细胞溶解早期建立的，但一直持续到淋巴瘤形成。相比之下，体液免疫首先在肠道系统发挥作用，并在细胞溶解后期开始发挥作用。GaHV2引起的神经损伤首先出现在细胞溶解的早期阶段，然后在很长一段时间内持续到随后的阶段。在增殖期，许多与转录和/或翻译相关的途径显著丰富，反映了细胞转化和淋巴瘤的形成。我们的工作为宿主对GaHV2感染的反应提供了一个全面的视角，并为进一步研究MD生物学提供了有意义的基础。
Gallid alphaherpesvirus 2 (GaHV2) is an oncogenic avian herpesvirus inducing Marek’s disease (MD) and rapid-onset T-cell lymphomas. To reveal molecular events in MD pathogenesis and tumorigenesis, the dynamic splenic transcriptome of GaHV2-infected chickens during early infection and pathogenic phases has been determined utilizing RNA-seq. Based on the significant differentially expressed genes (DEGs), analysis of gene ontology, KEGG pathway and protein-protein interaction network has demonstrated that the molecular events happening during GaHV2 infection are highly relevant to the disease course. In the ‘Cornell Model’ description of MD, innate immune responses and inflammatory responses were established at early cytolytic phase but persisted until lymphoma formation. Humoral immunity in contrast began to play a role firstly in the intestinal system and started at late cytolytic phase. Neurological damage caused by GaHV2 is first seen in early cytolytic phase and is then sustained throughout the following phases over a long time period. During the proliferative phase many pathways associated with transcription and/or translation were significantly enriched, reflecting the cell transformation and lymphoma formation. Our work provides an overall view of host responses to GaHV2 infection and offers a meaningful basis for further studies of MD biology.
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影响因子：
7.3
作者：
McLoughlin KE;Nalpas NC;Rue-Albrecht K;Browne JA;Magee DA;Killick KE;Park SD;Hokamp K;Meade KG;O'Farrelly C;Gormley E;Gordon SV;MacHugh DE
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影响因子：
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Pestova, TV
影响因子：
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作者：
Korneeva, NL;Lamphear, BJ;Rhoads, RE
通讯作者：
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影响因子：
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作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
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