Nanoparticle curcumin ameliorates experimental colitis via modulation of gut microbiota and induction of regulatory T cells.
Nanoparticle curcumin ameliorates experimental colitis via modulation of gut microbiota and induction of regulatory T cells.
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DOI：
10.1371/journal.pone.0185999
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Andoh A
中科院分区：
文献类型：
--
作者：
Ohno M;Nishida A;Sugitani Y;Nishino K;Inatomi O;Sugimoto M;Kawahara M;Andoh A
姜黄素是一种疏水性多酚，来源于姜黄，一种传统的印度香料。姜黄素具有多种生物学功能，但口服后吸收不良，限制了其临床应用。一种新开发的姜黄素纳米颗粒显示出改善的体内吸收性。在这项研究中，我们研究了纳米姜黄素（名为Theracurmin）对小鼠实验性结肠炎的影响。用3%葡聚糖硫酸钠（DSS）水溶液喂养BALB/c小鼠。实时荧光定量PCR和流式细胞术分别分析粘液细胞因子表达和淋巴细胞亚群。通过实时PCR分析肠道微生物群的概况。用纳米姜黄素治疗显著减轻体重减轻、疾病活动指数、组织学结肠炎评分并显著改善粘膜渗透性。免疫印迹分析显示，纳米姜黄素处理显著抑制结肠上皮细胞NF-κB的活化。姜黄素纳米颗粒治疗显著抑制了炎症介质的粘蛋白mRNA表达。用纳米姜黄素处理增加了产丁酸细菌的丰度和粪便丁酸水平。这伴随着结肠粘膜中CD 4 + Foxp 3+调节性T细胞和CD 103 + CD 8 α−调节性树突状细胞的扩增增加。纳米姜黄素治疗可能通过调节肠道微生物结构抑制DSS诱导的结肠炎的发展。这些反应与具有调节特性的粘膜免疫细胞的诱导有关。纳米颗粒姜黄素是治疗IBD的治疗选择的有前途的候选者之一。
Curcumin is a hydrophobic polyphenol derived from turmeric, a traditional Indian spice. Curcumin exhibits various biological functions, but its clinical application is limited due to its poor absorbability after oral administration. A newly developed nanoparticle curcumin shows improved absorbability in vivo. In this study, we examined the effects of nanoparticle curcumin (named Theracurmin) on experimental colitis in mice. BALB/c mice were fed with 3% dextran sulfate sodium (DSS) in water. Mucosal cytokine expression and lymphocyte subpopulation were analyzed by real-time PCR and flow cytometry, respectively. The profile of the gut microbiota was analyzed by real-time PCR. Treatment with nanoparticle curcumin significantly attenuated body weight loss, disease activity index, histological colitis score and significantly improved mucosal permeability. Immunoblot analysis showed that NF-κB activation in colonic epithelial cells was significantly suppressed by treatment with nanoparticle curcumin. Mucosal mRNA expression of inflammatory mediators was significantly suppressed by treatment with nanoparticle curcumin. Treatment with nanoparticle curcumin increased the abundance of butyrate-producing bacteria and fecal butyrate level. This was accompanied by increased expansion of CD4+ Foxp3+ regulatory T cells and CD103+ CD8α− regulatory dendritic cells in the colonic mucosa. Treatment with nanoparticle curcumin suppressed the development of DSS-induced colitis potentially via modulation of gut microbial structure. These responses were associated with induction of mucosal immune cells with regulatory properties. Nanoparticle curcumin is one of the promising candidates as a therapeutic option for the treatment of IBD.
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影响因子：
15.9
作者：
Eash, Kyle J.;Greenbaum, Adam M.;Link, Daniel C.
通讯作者：
Link, Daniel C.
影响因子：
29.4
作者：
Nishida A;Lau CW;Zhang M;Andoh A;Shi HN;Mizoguchi E;Mizoguchi A
通讯作者：
Mizoguchi A
影响因子：
4.4
作者：
Nagashima, K;Hisada, T;Mochizuki, J
通讯作者：
Mochizuki, J
影响因子：
12.6
作者：
Hanai, Hiroyuki;Iida, Takayuki;Koide, Yukio
通讯作者：
Koide, Yukio
影响因子：
12.2
作者：
Narushima S;Sugiura Y;Oshima K;Atarashi K;Hattori M;Suematsu M;Honda K
通讯作者：
Honda K