Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging.
Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging.
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DOI：
10.1021/bi2000966
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发表时间：
2011-04-05
期刊：
影响因子：
2.9
通讯作者：
Achilefu S
中科院分区：
文献类型：
--
作者：
Berezin MY;Guo K;Akers W;Livingston J;Solomon M;Lee H;Liang K;Agee A;Achilefu S
我们证明，通常用于标记小肽用于分子成像而不是结合的肽的碳酸染料的结构控制着从血管到组织的渗出速率。通过检查几个近红外（NIR）碳氨氨酸荧光团，我们证明了染料与白蛋白，模型血浆蛋白的结合与探针渗出速率之间的定量相关性。染料的结合是通过色氨酸在白蛋白中的荧光淬灭来测量的，并发现与渗出速率成反比。通过使用啮齿动物耳模型的纵向成像研究确定的峰度为定量测量提供了基础。旨在评估NIR荧光氰探针代表性文库的结构活性研究表明，具有结合常数的亲水性染料比其疏水性对应物低几个数量级具有更快的渗水速率，从而确立了理性探针设计的基础。该相关性提供了光学成像中染料选择的指南，以及一种验证特定染料是否最佳的方法的方法
We demonstrate that the structure of carbocyanine dyes, which are commonly used to label small peptides for molecular imaging, and not the bound peptide, controls the rate of extravasation from blood vessels to tissue. By examining several near-infrared (NIR) carbocyanine fluorophores, we demonstrate a quantitative correlation between the binding of a dye to albumin, a model plasma protein, and the rate of extravasation of the probe into tissue. Binding of the dyes was measured by fluorescence quenching of the tryptophans in albumin and was found to be inversely proportional to the rate of extravasation. The rate of extravasation, determined by kurtosis from longitudinal imaging studies using rodent ear models, provided a basis for quantitative measurements. Structure-activity studies aimed at evaluating a representative library of NIR fluorescent cyanine probes showed that hydrophilic dyes with binding constants several orders of magnitude lower than their hydrophobic counterparts have much faster extravasation rate, establishing a foundation for rational probe design. The correlation provides a guideline for dye selection in optical imaging and a method to verify if a certain dye is optimal for a specific molecular imaging application
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影响因子：
3.3
作者：
Berezin, Mikhail Y.;Lee, Hyeran;Achilefu, Samuel
通讯作者：
Achilefu, Samuel
DOI：
10.1073/pnas.0702427104
发表时间：
2007-05-29
影响因子：
11.1
作者：
de Chermont, Quentin le Masne;Chaneac, Corinne;Scherman, Daniel
通讯作者：
Scherman, Daniel
影响因子：
3.6
作者：
Lee, Hyeran;Mason, J. Christian;Achilefu, Samuel
通讯作者：
Achilefu, Samuel
DOI：
10.1016/j.jpba.2006.02.004
发表时间：
2006-08-28
影响因子：
3.4
作者：
Frazier, Richard A.;Papadopoulou, Athina;Green, Rebecca J.
通讯作者：
Green, Rebecca J.
影响因子：
4.8
作者：
Johansson, JS
通讯作者：
Johansson, JS