Salivary polypeptide/hyaluronic acid multilayer coatings act as "fungal repellents" and prevent biofilm formation on biomaterials.
Salivary polypeptide/hyaluronic acid multilayer coatings act as "fungal repellents" and prevent biofilm formation on biomaterials.
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DOI：
10.1039/c7tb02592k
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发表时间：
2018-03-14
影响因子：
7
通讯作者：
Sun, Yuyu
中科院分区：
文献类型：
--
作者：
Wen, Jianchuan;Yeh, Chih-Ko;Sun, Yuyu
念珠菌相关假牙口腔炎 (CADS) 是假牙佩戴者常见的、反复出现的临床并发症，可导致严重的口腔和全身健康问题。由于治疗效果短、效果差，目前的治疗策略并不令人满意。在这里，我们描述了一种新的真菌生物膜控制策略，在义齿材料上使用聚电解质逐层（LBL）自组装技术。传统的聚甲基丙烯酸甲酯 (PMMA) 义齿材料盘通过等离子体引发的表面接枝，用带负电的聚甲基丙烯酸 (PMAA) 进行功能化，然后用唾液抗菌多肽组蛋白 5（H-5；阳离子聚合物）和透明质酸（HA；阴离子聚合物）重复交替涂覆。另一方面，H-5/HA LBL涂层（即最外层为H-5）抑制真菌附着/粘附，显着减少真菌生物膜形成，并与抗真菌药物咪康唑表现出协同作用。 LBL 表面亲水性并不是控制念珠菌生物膜形成的关键机制。目前的方法展示了新设计原理在制造抗念珠菌义齿材料以及其他潜在相关医疗设备方面的实用性，可用于控制真菌生物膜的形成和对抗隐匿感染。
Candida-associated denture stomatitis (CADS) is a common, recurring clinical complication in denture wearers that can lead to serious oral and systemic health problems. Current management strategies are not satisfactory due to short-acting and ineffective therapeutic effects. Here, we describe a new fungal biofilm controlling strategy using polyelectrolyte layer-by-layer (LBL) self-assembly technology on denture materials. Conventional poly (methyl methacrylate) (PMMA) denture material discs were functionalized with negatively charged poly (methacrylic acid) (PMAA) via plasma-initiated surface grafting, followed by repetitive alternating coating with the salivary antimicrobial polypeptide histatin 5 (H-5; cationic polymer) and hyaluronic acid (HA; anionic polymer). On the other hand, the H-5/HA LBL coatings (i.e., the outermost layer was H-5) inhibited fungal attachment/adhesion, significantly reduced fungal biofilm formation, and showed synergistic effects with the antifungal drug miconazole. LBL surface hydrophilicity was not the key mechanism in controlling Candida biofilm formation. The current approach demonstrates the utility of a new design principle for fabricating anticandidal denture materials, as well as potentially other related medical devices, for controlling fungal biofilm formation and combating insidious infections.
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影响因子：
4.4
作者：
LAMB, DJ;DOUGLAS, CWI
通讯作者：
DOUGLAS, CWI
影响因子：
4.8
作者：
Helmerhorst, EJ;van't Hof, W;Oppenheim, FG
通讯作者：
Oppenheim, FG
DOI：
10.1016/0030-4220(90)90265-t
发表时间：
1990-01-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
EPSTEIN, JB
通讯作者：
EPSTEIN, JB
影响因子：
2.7
作者：
Pereira-Cenci, Tatiana;Del Bel Cury, Altair Antoninha;Ten Cate, Jacob Martien
通讯作者：
Ten Cate, Jacob Martien
影响因子：
6.2
作者：
Jia, Xinqiao;Yeo, Yoon;Langer, Robert
通讯作者：
Langer, Robert