Aliifodinibius roseus gen. nov., sp. nov., and Aliifodinibius sediminis sp. nov., two moderately halophilic bacteria isolated from salt mine samples.

Aliifodinibius roseus gen. nov., sp. nov., and Aliifodinibius sediminis sp. nov., two moderately halophilic bacteria isolated from salt mine samples.
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Aliifodinibius 玫瑰属。

DOI:
10.1099/ijs.0.043869-0
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发表时间:
2013-08
影响因子:
2.8
通讯作者:
Cui Xiao-Long
Cui Xiao-Long
中科院分区:
生物学3区
文献类型:
--
作者:
Li Zhi-Ying;Ji Kai-Yang;Wen Meng-Liang;Cui Xiao-Long

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从云南两个盐矿中分离到两种杆状非运动细菌。命名为YIM D15 T和YIM J21 T的这些菌株是革兰氏阴性和中度嗜盐的。两种菌株需要6- 10%NaCl(w/v;最佳)生长。YIM D15 T和YIM J21 T的DNA G+C含量分别为49.0mol%和48.4mol%。主要的类异戊二烯醌是MK-7。YIM D15 T和YIM J21 T的极性脂主要由二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、三种未知极性脂和一种糖脂组成。也可检测到少量其他脂质。主要的细胞脂肪酸为异-C15:0、反异-C15:0、异-C17:1ω 9 c/10甲基-C16:0和C16:1ω 7 c/C16:1ω6c。基于16 S rRNA基因序列比较的系统发育分析表明,这两个分离物与该属形成了一个独特的分支 福迪尼比乌斯 (in动物门 Bacteroidetes ),并与物种 盐沼弗氏脂鲤 同源性为91.9- 92.4%。16 S rRNA基因序列分析表明,YIM D15 T和YIM J21 T两个菌株之间存在一定的亲缘关系(97.3%的序列相似性)。两个分离物的DNA-DNA杂交相关性为34%。根据系统发育分析、DNA-DNA杂交相关性、表型特征和化学分类学特征,YIM D15 T和YIM J21 T菌株应被划分为一个新属的成员和两个新种,并命名为Aliifodinibius roseus gen. nov.,sp. nov.(模式菌株YIM D15 T = ACCC 10715 T = KCTC 23442 T)和Aliifodinibius sediminis sp. nov.(模式菌株YIM J21 T = ACCC 10714 T = DSM 21194 T)。
Two rod-shaped, non-motile bacteria were isolated from two separate salt mines in Yunnan, south-western China. These strains, designated YIM D15T and YIM J21T, were Gram-negative and moderately halophilic. The two strains required 6–10 % NaCl (w/v; optimal) for growth. The DNA G+C contents of strains YIM D15T and YIM J21T were 49.0 mol% and 48.4 mol%, respectively. The predominant isoprenoid quinone was MK-7. The polar lipid profiles of strains YIM D15T and YIM J21T were composed predominantly of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unknown polar lipids and one glycolipid. Minor amounts of other lipids were also detectable. The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 1ω9c/10 methyl-C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the two isolates formed a distinct clade with the genus Fodinibius (in the phylum Bacteroidetes ) and were related to the species Fodinibius salinus , with sequence similarities of 91.9–92.4 %. Analyses of 16S rRNA gene sequences revealed that strains YIM D15T and YIM J21T were related to each other (97.3 % sequence similarity). The DNA–DNA hybridization relatedness between the two isolates was 34 %. On the basis of the phylogenetic analysis, DNA–DNA hybridization relatedness, phenotypic and chemotaxonomic characteristics, strains YIM D15T and YIM J21T should be classified as members of a novel genus and as two novel species, for which the names Aliifodinibius roseus gen. nov., sp. nov. (type strain YIM D15T = ACCC 10715T = KCTC 23442T) and Aliifodinibius sediminis sp. nov. (type strain YIM J21T = ACCC 10714T = DSM 21194T) are proposed.
DOI: 10.1038/178703a0
发表时间: 1956-01-01
期刊: NATURE
影响因子: 64.8
作者:
KOVACS, N
通讯作者: KOVACS, N
DOI: 10.1002/0471650129.dob0534
发表时间: 2004-10
期刊: Dictionary of Bioinformatics and Computational Biology
影响因子: --
作者:
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DOI: 10.2172/205047
发表时间: 1996-03
影响因子: 3.2
作者:
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通讯作者: G. Olsen;C. Woese;R. Overbeek
DOI: --
发表时间: 1994-10
期刊: --
影响因子: --
作者:
R. Murray;R. Doetsch;C. Robinow
通讯作者: R. Murray;R. Doetsch;C. Robinow
DOI: 10.1099/00221287-100-2-221
发表时间: 1977-01-01
期刊: JOURNAL OF GENERAL MICROBIOLOGY
影响因子: --
作者:
COLLINS, MD;PIROUZ, T;MINNIKIN, DE
通讯作者: MINNIKIN, DE