Timed exercise stabilizes behavioral rhythms but not molecular programs in the brain's suprachiasmatic clock.
Timed exercise stabilizes behavioral rhythms but not molecular programs in the brain's suprachiasmatic clock.
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DOI：
10.1016/j.isci.2023.106002
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发表时间：
2023-02-17
期刊：
影响因子：
5.8
通讯作者：
Piggins, Hugh D.
中科院分区：
文献类型：
--
作者：
Hitrec, Timna;Petit, Cheryl;Cryer, Emily;Muir, Charlotte;Tal, Natalie;Fustin, Jean-Michel;Hughes, Alun T. L.;Piggins, Hugh D.
每天定时使用跑轮（预定的自愿运动；SVE）可以同步啮齿类动物的昼夜节律，并促进神经肽信号传导基因靶向损伤的动物（Vipr2−/− 小鼠）保持稳定的 24 小时节律。在这里，我们使用 RNA-seq 和/或 qRT-PCR 来评估这种神经肽信号传导损伤以及 SVE 如何塑造大脑时钟（视交叉上核；SCN）和外周组织（肝脏和肺）中的分子程序。与 Vipr2+/+ 动物相比，Vipr2−/− 小鼠的 SCN 转录组显示出广泛的失调，包括核心时钟成分、转录因子和神经化学物质。此外，尽管 SVE 稳定了这些动物的行为节律，但 SCN 转录组仍然失调。 Vipr2−/− 小鼠肺和肝脏中的分子程序部分完整，尽管它们对 SVE 的反应与 Vipr2+/+ 小鼠中这些外周组织的反应不同。这些发现强调，SVE 可以纠正昼夜节律的行为异常，而不会对 SCN 转录组造成大规模改变。定时运动可促进 VPAC2 缺陷小鼠的稳定行为节律 VPAC2 的缺失会使视交叉上 (SCN) 转录组失调 定时运动不会恢复 VPAC2 缺陷小鼠的 SCN 转录组 定时运动的行为影响需要功能性分子时钟 分子生物学；神经科学；行为神经科学
Timed daily access to a running-wheel (scheduled voluntary exercise; SVE) synchronizes rodent circadian rhythms and promotes stable, 24h rhythms in animals with genetically targeted impairment of neuropeptide signaling (Vipr2−/− mice). Here we used RNA-seq and/or qRT-PCR to assess how this neuropeptide signaling impairment as well as SVE shapes molecular programs in the brain clock (suprachiasmatic nuclei; SCN) and peripheral tissues (liver and lung). Compared to Vipr2+/+ animals, the SCN transcriptome of Vipr2−/− mice showed extensive dysregulation which included core clock components, transcription factors, and neurochemicals. Furthermore, although SVE stabilized behavioral rhythms in these animals, the SCN transcriptome remained dysregulated. The molecular programs in the lung and liver of Vipr2−/− mice were partially intact, although their response to SVE differed to that of these peripheral tissues in the Vipr2+/+ mice. These findings highlight that SVE can correct behavioral abnormalities in circadian rhythms without causing large scale alterations to the SCN transcriptome. Timed exercise promotes stable behavioral rhythms in VPAC2 deficient mice Loss of VPAC2 dysregulates the suprachiasmatic (SCN) transcriptome Timed exercise does not restore the SCN transcriptome in VPAC2 deficient mice Behavioral effects of timed exercise require a functional molecular clock Molecular biology; Neuroscience; Behavioral neuroscience
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影响因子：
64.8
作者：
Alenghat, Theresa;Meyers, Katherine;Mullican, Shannon E.;Leitner, Kirstin;Adeniji-Adele, Adetoun;Avila, Jacqueline;Bucan, Maja;Ahima, Rexford S.;Kaestner, Klaus H.;Lazar, Mitchell A.
通讯作者：
Lazar, Mitchell A.
影响因子：
64.8
作者：
Cheng, MY;Bullock, CM;Zhou, QY
通讯作者：
Zhou, QY
影响因子：
3.7
作者：
Chang SW;Yoshihara T;Tsuzuki T;Natsume T;Kakigi R;Machida S;Naito H
通讯作者：
Naito H
影响因子：
7.7
作者：
Araki, Yoichi;Hong, Ingie;Huganir, Richard L.
通讯作者：
Huganir, Richard L.
DOI：
10.1126/science.1198125
发表时间：
2011-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng D;Liu T;Sun Z;Bugge A;Mullican SE;Alenghat T;Liu XS;Lazar MA
通讯作者：
Lazar MA