Three‐dimensional reconstruction of the cranial and anterior spinal nerves in early tadpoles of Xenopus laevis (Pipidae, Anura)

Three‐dimensional reconstruction of the cranial and anterior spinal nerves in early tadpoles of Xenopus laevis (Pipidae, Anura)
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非洲爪蟾(Pipidae,Anura)早期蝌蚪颅神经和前脊神经的三维重建

DOI:
10.1002/cne.24370
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发表时间:
2017
影响因子:
2.5
通讯作者:
Olsson
Olsson
中科院分区:
医学3区
文献类型:
--
作者:
Naumann;Olsson

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非洲爪蟾是神经生物学中应用最广泛的模式生物之一。因此,令人惊讶的是,没有详细和完整的描述脑神经存在这个物种。使用经典的组织学切片结合荧光整体抗体染色和微型计算机断层扫描,我们制备了早期X的颅神经和最前部脊神经的详细神经支配图和可自由旋转的三维(3D)模型。紫菜我们的研究结果证实了早期对耳前颅神经的描述,并首次详细描述了耳后颅神经。追踪神经支配,我们在X中发现了两个以前未描述的头部肌肉(关节突肌和鳃肌)。光滑。关于蝌蚪脑神经形态的数据很少,只有一个其他物种(Discoglossus pictus)被描述得非常详细。Xenopus和Discoglossus的比较揭示了耳后颅神经的相对保守的模式和耳前颅神经的更多变的形态。此外,这里提供的神经支配图和3D模型可以作为一个易于访问的基础,以识别由实验研究(如遗传获得和功能丧失实验)产生的神经支配的改变。
Xenopus laevisis one of the most widely used model organism in neurobiology. It is therefore surprising, that no detailed and complete description of the cranial nerves exists for this species. Using classical histological sectioning in combination with fluorescent whole mount antibody staining and micro‐computed tomography we prepared a detailed innervation map and a freely‐rotatable three‐dimensional (3D) model of the cranial nerves and anterior‐most spinal nerves of earlyX. laevistadpoles. Our results confirm earlier descriptions of the pre‐otic cranial nerves and present the first detailed description of the post‐otic cranial nerves. Tracing the innervation, we found two previously undescribed head muscles (the processo‐articularis and diaphragmatico‐branchialis muscles) inX. laevis. Data on the cranial nerve morphology of tadpoles are scarce, and only one other species (Discoglossus pictus) has been described in great detail. A comparison ofXenopusandDiscoglossusreveals a relatively conserved pattern of the post‐otic and a more variable morphology of the pre‐otic cranial nerves. Furthermore, the innervation map and the 3D models presented here can serve as an easily accessible basis to identify alterations of the innervation produced by experimental studies such as genetic gain‐ and loss of function experiments.
DOI: --
发表时间: --
期刊:
影响因子: --
作者:
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DOI: 10.1242/dmm.012138
发表时间: 2013-09
影响因子: 4.3
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非洲爪蟾蝌蚪发育中肌膜中交界外乙酰胆碱受体的快速横向扩散
DOI: 10.1523/jneurosci.03-01-00225.1983
发表时间: 1983
期刊: Angewandte Chemie
影响因子: --
作者:
S. H. Young;M. Poo
通讯作者: M. Poo
DOI: 10.1016/j.tig.2011.08.003
发表时间: 2011-12
期刊: TRENDS IN GENETICS
影响因子: 11.4
作者:
Harland, Richard M.;Grainger, Robert M.
通讯作者: Grainger, Robert M.
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DOI: --
发表时间: 1983
期刊: The Journal of comparative neurology
影响因子: --
作者:
J. Altman;E. Dawes
通讯作者: E. Dawes