Stimuli-responsive vesicles as distributed artificial organelles for bacterial activation.
Stimuli-responsive vesicles as distributed artificial organelles for bacterial activation.
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DOI：
10.1073/pnas.2206563119
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发表时间：
2022-10-18
影响因子：
11.1
通讯作者：
Weitz, David
中科院分区：
文献类型：
--
作者：
Gispert, Ignacio;Hindley, James W.;Pilkington, Colin P.;Shree, Hansa;Barter, Laura M. C.;Ces, Oscar;Elani, Yuval;Weitz, David
人造细胞是旨在模仿生物细胞的形式和功能的实体。该领域的重大挑战之一是设计“非生命”人造细胞，使其与生物“生命”对应物进行通信。将定制人造细胞的可编程性与生物细胞的生物技术能力相结合，有可能支撑生物技术和医学的应用。通过实现生物细胞的按需外部控制激活，人造细胞构成可编程模块，将物理输入转化为细菌响应的化学信号。至关重要的是，细菌无需经过基因工程即可获得扩展的感官范围。这为组装人造细胞器铺平了道路，并为使用人造细胞作为治疗应用及其他领域的工具开辟了新途径。细胞间通讯是生命系统的标志。因此，工程化具有这种行为的人造细胞一直是自下而上的合成生物学活动的核心。人工细胞和活细胞之间的通讯有可能赋予活有机体新的能力，这些能力可用于生物医学和生物技术。然而，目前大多数方法依赖于无法外部控制的化学信号交换。在这里，我们报告了两种类型的远程控制的基于囊泡的人工细胞器，它们将物理输入转化为导致细菌激活的化学信息。在光或温度刺激下，人造细胞膜被激活，释放信号分子，诱导大肠杆菌中的蛋白质表达。这种分布式方法不同于现有的刺激响应细菌工程方法。在这里，人造细胞（而不是细菌细胞本身）是设计单位。将刺激响应元件划分在人造细胞中在治疗、组织工程和生物修复方面具有潜在的应用。它将支持混合生物/非生物系统的设计，在该系统中可以对种群相互作用进行时间控制。
Artificial cells are entities designed to mimic biological cells in form and function. One of the grand challenges of this field is engineering “nonliving” artificial cells to communicate with their biological “living” counterparts. Combining the programmability of tailor-made artificial cells with the biotechnological power of biological cells has the potential to underpin applications in biotechnology and medicine. By achieving on-demand externally controlled activation of biological cells, the artificial cells constitute programmable modules that translate physical inputs into chemical signals that bacteria respond to. Crucially, the bacteria gain an extended sensory range without undergoing genetic engineering. This paves the way toward assembling artificial organelles and opens up new avenues in using artificial cells as tools in therapeutic applications and beyond. Intercellular communication is a hallmark of living systems. As such, engineering artificial cells that possess this behavior has been at the heart of activities in bottom-up synthetic biology. Communication between artificial and living cells has potential to confer novel capabilities to living organisms that could be exploited in biomedicine and biotechnology. However, most current approaches rely on the exchange of chemical signals that cannot be externally controlled. Here, we report two types of remote-controlled vesicle-based artificial organelles that translate physical inputs into chemical messages that lead to bacterial activation. Upon light or temperature stimulation, artificial cell membranes are activated, releasing signaling molecules that induce protein expression in Escherichia coli. This distributed approach differs from established methods for engineering stimuli-responsive bacteria. Here, artificial cells (as opposed to bacterial cells themselves) are the design unit. Having stimuli-responsive elements compartmentalized in artificial cells has potential applications in therapeutics, tissue engineering, and bioremediation. It will underpin the design of hybrid living/nonliving systems where temporal control over population interactions can be exerted.
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影响因子：
5
作者：
Grimes PJ;Galanti A;Gobbo P
通讯作者：
Gobbo P
影响因子：
4.6
作者：
Elani Y;Trantidou T;Wylie D;Dekker L;Polizzi K;Law RV;Ces O
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Ces O
影响因子：
4.4
作者：
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通讯作者：
Needham, D
DOI：
10.3390/life4041092
发表时间：
2014-12-19
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Ding Y;Wu F;Tan C
通讯作者：
Tan C
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影响因子：
5.4
作者：
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通讯作者：
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