Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.
Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.
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DOI：
10.1186/gb-2010-11-12-r119
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发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Shendure J
中科院分区：
文献类型：
--
作者：
Adey A;Morrison HG;Asan;Xun X;Kitzman JO;Turner EH;Stackhouse B;MacKenzie AP;Caruccio NC;Zhang X;Shendure J
我们的特点和扩展一个高效的方法构建鸟枪片段库，其中转座酶催化体外DNA片段化和衔接子掺入的同时。我们将这种方法应用于人类基因组测序，发现覆盖偏差与传统协议的覆盖偏差相当。我们还通过开发用于亚纳克文库构建、从50 ng输入DNA捕获外显子组、无PCR和菌落PCR文库构建以及96重样品索引的方案来扩展其功能。
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.
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影响因子：
48
作者：
Craig, David W.;Pearson, John V.;Szelinger, Szabolcs;Sekar, Aswin;Redman, Margot;Corneveaux, Jason J.;Pawlowski, Traci L.;Laub, Trisha;Nunn, Gary;Stephan, Dietrich A.;Homer, Nils;Huentelman, Matthew J.
通讯作者：
Huentelman, Matthew J.
影响因子：
5.6
作者：
SCHNEIDER, TD;STORMO, GD;EHRENFEUCHT, A
通讯作者：
EHRENFEUCHT, A
DOI：
10.1073/pnas.82.23.8129
发表时间：
1985-01-01
影响因子：
11.1
作者：
MANOIL, C;BECKWITH, J
通讯作者：
BECKWITH, J
DOI：
10.1073/pnas.95.18.10716
发表时间：
1998-09-01
影响因子：
11.1
作者：
Goryshin, IY;Miller, JA;Reznikoff, WS
通讯作者：
Reznikoff, WS
亚洲个体的二倍体基因组序列
影响因子：
64.8
作者：
Wang, Jun;Wang, Wei;Li, Ruiqiang;Li, Yingrui;Tian, Geng;Goodman, Laurie;Fan, Wei;Zhang, Junqing;Li, Jun;Zhang, Juanbin;Guo, Yiran;Feng, Binxiao;Li, Heng;Lu, Yao;Fang, Xiaodong;Liang, Huiqing;Du, Zhenglin;Li, Dong;Zhao, Yiqing;Hu, Yujie;Yang, Zhenzhen;Zheng, Hancheng;Hellmann, Ines;Inouye, Michael;Pool, John;Yi, Xin;Zhao, Jing;Duan, Jinjie;Zhou, Yan;Qin, Junjie;Ma, Lijia;Li, Guoqing;Yang, Zhentao;Zhang, Guojie;Yang, Bin;Yu, Chang;Liang, Fang;Li, Wenjie;Li, Shaochuan;Li, Dawei;Ni, Peixiang;Ruan, Jue;Li, Qibin;Zhu, Hongmei;Liu, Dongyuan;Lu, Zhike;Li, Ning;Guo, Guangwu;Zhang, Jianguo;Ye, Jia;Fang, Lin;Hao, Qin;Chen, Quan;Liang, Yu;Su, Yeyang;San, A.;Ping, Cuo;Yang, Shuang;Chen, Fang;Li, Li;Zhou, Ke;Zheng, Hongkun;Ren, Yuanyuan;Yang, Ling;Gao, Yang;Yang, Guohua;Li, Zhuo;Feng, Xiaoli;Kristiansen, Karsten;Wong, Gane Ka-Shu;Nielsen, Rasmus;Durbin, Richard;Bolund, Lars;Zhang, Xiuqing;Li, Songgang;Yang, Huanming;Wang, Jian
通讯作者：
Wang, Jian