Effects of Pinealectomy and Short Day Lengths on Reproduction and Neuronal RFRP-3, Kisspeptin, and GnRH in Female Turkish Hamsters.
Effects of Pinealectomy and Short Day Lengths on Reproduction and Neuronal RFRP-3, Kisspeptin, and GnRH in Female Turkish Hamsters.
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DOI：
10.1177/0748730414532423
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发表时间：
2014-06
影响因子：
3.5
通讯作者：
Kriegsfeld LJ
中科院分区：
文献类型：
--
作者：
Piekarski DJ;Jarjisian SG;Perez L;Ahmad H;Dhawan N;Zucker I;Kriegsfeld LJ
长日照（LDs）刺激和短日照（SDs）抑制光周期啮齿类动物的繁殖，通过修改夜间松果体褪黑激素分泌。在LD土耳其仓鼠，不像其他啮齿类动物，松果体切除术诱导生殖静止相媲美的SD。我们评估了SD和松果体切除术是否诱导类似或不同模式的kisspeptin和促性腺激素抑制激素（在哺乳动物中也称为RFamide相关肽-3 [RFRP-3]）表达，这些表达是其他物种季节性生殖变化的重要介质。从保持在LD和SD中的假手术雌性土耳其仓鼠和LD-切除（pinx）雌性中收获脑，所有这些仓鼠均在其各自的光周期中饲养12周。LD-假手术组的子宫重量显著高于LD-pinx和SD-假手术组雌性动物。RFRP-3-免疫反应（IR）细胞在背内侧下丘脑核的数量和大小在繁殖能力的LD-假仓鼠比繁殖抑制SD-假和LD-pinx仓鼠。LD-假仓鼠有更多的kisspeptin-ir细胞在前腹侧室周核比LD-pinx仓鼠。生殖静止，无论是由短日照长度或松果体切除术引起的，通常伴随着RFRP-3和kisspeptin的可比变化，这表明长时间的褪黑激素信号和松果体切除术的褪黑激素撤退可能会通过相同的神经底物诱导性腺静止。
Long days (LDs) stimulate and short days (SDs) inhibit reproduction in photoperiodic rodents by modifying nocturnal pineal melatonin secretion. In LD Turkish hamsters, unlike other rodents, pinealectomy induces reproductive quiescence comparable to that produced by SDs. We assessed whether SDs and pinealectomy induce similar or different patterns of kisspeptin and gonadotropin-inhibitory hormone (also known as RFamide-related peptide-3 [RFRP-3] in mammals) expression, important mediators of seasonal reproductive changes in other species. Brains were harvested from sham-operated female Turkish hamsters maintained in LDs and SDs and LD-pealectomized (pinx) females, all housed in their respective photoperiods for 12 weeks. Uterine weights were substantially higher in LD-sham than in LD-pinx and SD-sham females. RFRP-3-immunoreactive(-ir) cells in the dorsomedial hypothalamic nucleus were greater in number and size in the reproductively competent LD-sham hamsters than in both reproductively suppressed SD-sham and LD-pinx hamsters. LD-sham hamsters had more kisspeptin-ir cells in the anteroventral periventricular nucleus than did LD-pinx hamsters. Reproductive quiescence, whether induced by short-day lengths or pinealectomy, was generally accompanied by comparable changes in RFRP-3 and kisspeptin, suggesting that long-duration melatonin signaling and withdrawal of melatonin by pinealectomy may act through the same neural substrates to induce gonadal quiescence.
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影响因子：
4.8
作者：
Greives, Timothy J.;Mason, Alex O.;Demas, Gregory E.
通讯作者：
Demas, Gregory E.
影响因子：
4.8
作者：
CARTER, DS;GOLDMAN, BD
通讯作者：
GOLDMAN, BD
DOI：
10.1073/pnas.1834399100
发表时间：
2003-09-16
影响因子：
11.1
作者：
de Roux, N;Genin, E;Milgrom, E
通讯作者：
Milgrom, E
影响因子：
3.2
作者：
Caraty, A.;Blomenroehr, M.;Beltramo, M.
通讯作者：
Beltramo, M.
影响因子：
3.6
作者：
HALL, VD;BARTKE, A;GOLDMAN, BD
通讯作者：
GOLDMAN, BD