Conversion of the sensor kinase DcuS of Escherichia coli of the DcuB/DcuS sensor complex to the C4 -dicarboxylate responsive form by the transporter DcuB.
Conversion of the sensor kinase DcuS of Escherichia coli of the DcuB/DcuS sensor complex to the C4 -dicarboxylate responsive form by the transporter DcuB.
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通过转运蛋白 DcuB 将 DcuB/DcuS 传感器复合物的大肠杆菌的传感器激酶 DcuS 转化为 C4 -二羧酸响应形式
DOI：
10.1111/1462-2920.13418
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发表时间：
2016
影响因子：
5.1
通讯作者：
Unden G
中科院分区：
文献类型：
--
作者：
Wörner S;Strecker;Monzel C;Zeltner M;Witan J;Ebert-Jung;Unden G
大肠杆菌的传感器激酶DcuS在需氧条件下与C4-二羧酸转运蛋白DctA一起作用，形成DctA/DcuS传感器复合物。在厌氧条件下，富马酸呼吸中的C4-二羧酸转运由C4-二羧酸/富马酸反向转运蛋白DcuB催化。(i)DcuB与DcuS相互作用，如细菌双杂交系统（BACTH）和溶解膜蛋白的共色谱（mHPINE测定）所示。(ii)在DcuB/DcuS复合物中，仅DcuS充当传感器，因为DcuS的底物位点中的突变改变了传感的底物特异性，并且底物马来酸盐或3-硝基丙酸盐诱导DcuS响应而不影响DcuB的富马酸盐位点。(iii)富马酸盐诱导DcuS的半数最大浓度（1 - 2 mM）和相应的转运Km（50 µM）相差20 - 40倍。因此，富马酸盐位点在转运和感知方面不同。(iv)增加DcuB水平将DcuS从永久ON（DcuB缺乏）状态转化为富马酸盐响应形式。总体而言，数据显示DcuS和DcuB形成代表C4-二羧酸响应形式的DcuB/DcuS复合物，并且复合物的感觉位点位于DcuS中，而DcuB是将DcuS转化为感觉感受态所需的。
The sensor kinase DcuS ofEscherichia colico‐operates under aerobic conditions with the C4‐dicarboxylate transporter DctA to form the DctA/DcuS sensor complex. Under anaerobic conditions C4‐dicarboxylate transport in fumarate respiration is catalyzed by C4‐dicarboxylate/fumarate antiporter DcuB. (i) DcuB interacted with DcuS as demonstrated by a bacterial two‐hybrid system (BACTH) and by co‐chromatography of the solubilized membrane‐proteins (mHPINE assay). (ii) In the DcuB/DcuS complex only DcuS served as the sensor since mutations in the substrate site of DcuS changed substrate specificity of sensing, and substrates maleate or 3‐nitropropionate induced DcuS response without affecting the fumarate site of DcuB. (iii) The half‐maximal concentration for induction of DcuS by fumarate (1 to 2 mM) and the correspondingKmfor transport (50 µM) differ by a factor of 20 to 40. Therefore, the fumarate sites are different in transport and sensing. (iv) Increasing levels of DcuB converted DcuS from the permanent ON (DcuB deficient) state to the fumarate responsive form. Overall, the data show that DcuS and DcuB form a DcuB/DcuS complex representing the C4‐dicarboxylate responsive form, and that the sensory site of the complex is located in DcuS whereas DcuB is required for converting DcuS to the sensory competent state.
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影响因子：
3.2
作者：
Davies, SJ;Golby, P;Andrews, SC
通讯作者：
Andrews, SC
影响因子：
3.4
作者：
V. Müller;P. Jungblut;T. Meyer;S. Hunke
通讯作者：
V. Müller;P. Jungblut;T. Meyer;S. Hunke
影响因子：
16.8
作者：
Etzkorn, Manuel;Kneuper, Holger;Baldus, Marc
通讯作者：
Baldus, Marc
大肠杆菌 DctA/DcuS 传感器复合物中 DctA 和 DcuS 功能的区分：DctA 作为活性开关的功能和 DcuS 作为 C4-二羧酸盐传感器的功能
影响因子：
3.6
作者：
P. Steinmetz;Sebastian Wörner;G. Unden
通讯作者：
G. Unden
影响因子：
3.2
作者：
Kraemer, J.;Fischer, J. D.;Unden, G.
通讯作者：
Unden, G.