Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment.
Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment.
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局部给予冷冻保存的活微粉化羊膜可通过调节局部微环境加速糖尿病小鼠的伤口愈合。
DOI：
10.1016/j.biomaterials.2016.10.031
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发表时间：
2017
期刊：
影响因子：
14
通讯作者：
Xia Zhaofan
中科院分区：
文献类型：
--
作者：
Zheng Yongjun;Ji Shizhao;Wu Haibin;Tian Song;Zhang Yunqing;Wang Li;Fang He;Luo Pengfei;Wang Xingtong;Hu Xiaoyan;Xiao Shichu;Xia Zhaofan
大约25%的糖尿病患者终生患有糖尿病下肢溃疡，7%-20%的患者最终需要截肢，尽管进行了标准护理治疗。迫切需要开发治疗糖尿病伤口的新疗法。在这项研究中，我们使用冷冻保存的活的微粉化羊膜（300-600 μm）治疗糖尿病小鼠的伤口。解冻后的微粉化羊膜保留了高细胞活力，以及完整的细胞形态和膜结构。当移植到db/db小鼠的伤口上时，冷冻保存的活微粒化羊膜主要通过分泌生长、炎症和趋化性相关因子来显著促进糖尿病小鼠的伤口愈合，这些因子调节巨噬细胞迁移和表型转换，募集CD 34+祖细胞，并增加新血管形成。此外，微粉化羊膜基质可存在于真皮中并作为长期真皮支架。这些结果证明了冷冻保存的活微粒化羊膜作为即用型活真皮替代物的潜力，其解决了受损伤口的多种缺陷生理过程，以在临床上治疗糖尿病溃疡和其他慢性伤口。
Approximately 25% of diabetic patients suffer from diabetic lower-extremity ulcer throughout their lives and 7%–20% of patients will eventually need an amputation despite standard care treatment. The development of new therapies to treat diabetic wounds is urgent. In this study, we used cryopreserved living micronized amnion (300–600 μm) to treat wounds in diabetic mice. Post-thaw micronized amnion retained high cell viability, as well as intact cell morphology and membrane structure. When transplanted onto the wounds of db/db mice, the cryopreserved living micronized amnion greatly promoted wound healing in diabetic mice mainly by secreting growth, inflammation, and chemotaxis-related factors that regulated macrophage migration and phenotype switch, recruited CD34+progenitor cells, and increased neovascularization. In addition, the micronized amnion matrix can exist in the dermis and serve as a long-term dermal scaffold. These results demonstrated the potential of the cryopreserved living micronized amnion as a ready-to-use living dermal substitute that addresses multiple defective physiological processes of impaired wounds to treat diabetic ulcers and other chronic wounds in clinics.
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影响因子：
14
作者：
Jain, Shardool;Thanh-Huyen Tran;Amiji, Mansoor
通讯作者：
Amiji, Mansoor
DOI：
10.1093/humrep/deq040
发表时间：
2010-05
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Holm F;Ström S;Inzunza J;Baker D;Strömberg AM;Rozell B;Feki A;Bergström R;Hovatta O
通讯作者：
Hovatta O
影响因子：
14.6
作者：
J. Gruss;D. Jirsch
通讯作者：
J. Gruss;D. Jirsch
DOI：
--
发表时间：
1965-05
期刊：
The Lancet
影响因子：
--
作者：
Pask Ea;Lunn Jn
通讯作者：
Pask Ea;Lunn Jn
影响因子：
3.1
作者：
Sheikh ES;Sheikh ES;Fetterolf DE
通讯作者：
Fetterolf DE