Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus.
Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus.
复制标题
大鲵胃固有层的特洛细胞
DOI：
10.1038/srep33554
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发表时间：
2016-09-15
影响因子：
4.6
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Zhang H;Zhong S;Yu P;Ge T;Peng S;Guo X;Zhou Z
本研究采用光镜、免疫组织化学和透射电子显微镜等方法，对中国大蜥蜴的胃端细胞进行了鉴定。甲苯胺蓝染色显示TCS有1~2个位于胃固有层的极细极长极(TP)。TPS具有特有的结构，包括窝、足和二分枝。免疫组织化学显示CD34+/PDGFRCD34+/α+Tcs存在于间质中，呈串珠状，靠近胃腺和血管。透射电子显微镜也显示TCS存在于胃腺间质中。TCS/TPS位于胃腺、血管、内分泌细胞和干细胞附近。特别是，TPS经常包围干细胞。TCS与TPS、TPS与干细胞建立了密切联系。此外，在TCS/TPS附近也发现了外切体。我们的数据证实了TCS在两栖动物胃固有层中的存在，并提示TCS与常驻干细胞合作，调节内分泌细胞和胃腺的再生和动态平衡。
In this study, we attempt to identify gastric telocytes (TCs) of the Chinese giant salamander Andrias davidianus, by light microscopy, immunohistochemistry and transmission electron microscopy (TEM) methods. Toluidine blue staining showed TCs with one to two very thin and long telopodes (Tps) that were located in gastric lamina propria. Tps had characteristic structures, including podoms, podomers and dichotomous branching. Immunohistochemistry showed the existence of CD34+/PDGFRα+ TCs with moniliform Tps in stroma and were close to gastric glands and blood vessels. TEM micrographs also demonstrated the presence of TCs in interstitium between gastric glands. TCs/Tps were located in close proximity to gastric glands, blood vessels, endocrine cells and stem cells. In particular, Tps frequently surrounded stem cells. TCs and Tps, Tps and stem cells established close contacts. Moreover, the exosomes were also found near TCs/Tps. Our data confirmed the presence of TCs in gastric lamina propria of the amphibian, and suggested that TCs cooperate with resident stem cells to regulate endocrine cells and gastric glands regeneration and homeostasis.
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影响因子：
3.6
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
通讯作者：
Popescu, Laurentiu M.
影响因子：
5.3
作者：
Cantarero Carmona I;Luesma Bartolomé MJ;Junquera Escribano C
通讯作者：
Junquera Escribano C
影响因子：
5.3
作者：
Popescu LM;Fertig ET;Gherghiceanu M
通讯作者：
Gherghiceanu M
影响因子：
5.3
作者：
Cismaşiu VB;Popescu LM
通讯作者：
Popescu LM
影响因子：
5.3
作者：
Alunno A;Ibba-Manneschi L;Bistoni O;Rosa I;Caterbi S;Gerli R;Manetti M
通讯作者：
Manetti M