Telocytes in pleura: two- and three-dimensional imaging by transmission electron microscopy.
Telocytes in pleura: two- and three-dimensional imaging by transmission electron microscopy.
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DOI：
10.1007/s00441-010-1095-0
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发表时间：
2011-02
影响因子：
3.6
通讯作者：
Popescu, Laurentiu M.
中科院分区：
文献类型：
--
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
有关支持胸膜间皮的结缔（间质）细胞的超微结构的信息很少。我们的目的是检查端细胞（TC）是否存在于胸膜，在心外膜和肠系膜。TC是一种独特的细胞类型，其特征在于称为端足细胞（Tp）的特异性双链体。我们已经使用透射电子显微镜（TEM）和电子断层扫描（ET），以确定是否接受TC的超微结构诊断标准是由小鼠和人胸膜中的亚间皮层中存在的任何细胞亚群满足。利用透射电子显微镜对TC进行了表征。由于足粒（小于0.2 μm的细节）和足粒（容纳小窝、线粒体和内质网的小扩张）的交替，Tp呈长而念珠状。Tp分支遵循二分模式，并建立专门的细胞间连接复合物。TC，通过他们的TP，似乎形成了间皮下的间隙网络，覆盖约三分之二的近腔间皮层。ET揭示了复杂的连接结构和紧密连接连接胸膜TC，以及Tp中该水平的小泡。因此，胸膜TC与其他浆膜中描述的TC具有显著的相似性。TC是否是间皮细胞诱导的组织修复的（主要）参与者仍有待确定。然而，它们形成的极细长的Tp和复杂的连接结构以及囊泡（或外来体）的释放表明TC参与长距离同源或异源细胞通信。本文的在线版本（doi：10.1007/s 00441 -010-1095-0）包含补充材料，可供授权用户使用。
Information about the ultrastructure of connective (interstitial) cells supporting the pleural mesothelium is scarce. Our aim has been to examine whether telocytes (TCs) are present in pleura, as in epicardium and mesentery. TCs are a distinct type of cell, characterized by specific prolongations named telopodes (Tp). We have used transmission electron microscopy (TEM) and electron tomography (ET) to determine whether ultrastructural diagnostic criteria accepted for TCs are fulfilled by any of the cell subpopulations existing in the sub-mesothelial layer in mouse and human pleura. TCs have been identified with TEM by their characteristic prolongations. Tp appear long and moniliform, because of the alternation of podomeres (thin segments of less than 0.2 μm) and podoms (small dilations accommodating caveolae, mitochondria, and endoplasmic reticulum). Tp ramifications follow a dichotomic pattern and establish specialized cell-to-cell junctional complexes. TCs, via their Tp, seem to form an interstitial network beneath the mesothelium, covering about two-thirds of the abluminal mesothelial layer. ET has revealed complex junctional structures and tight junctions connecting pleural TCs, and small vesicles at this level in Tp. Thus, pleural TCs share significant similarities with TCs described in other serosae. Whether TCs are a (major) player in mesothelial-cell-induced tissue repair remains to be established. Nevertheless, the extremely long thin Tp and complex junctional structures that they form and the release of vesicles (or exosomes) indicate the participation of TCs in long-distance homo- or heterocellular communication. The online version of this article (doi:10.1007/s00441-010-1095-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.1582-4934.2006.tb00410.x
发表时间：
2006-04
影响因子：
5.3
作者：
Popescu LM;Gherghiceanu M;Hinescu ME;Cretoiu D;Ceafalan L;Regalia T;Popescu AC;Ardeleanu C;Mandache E
通讯作者：
Mandache E
DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
5.3
作者：
Cantarero Carmona I;Luesma Bartolomé MJ;Junquera Escribano C
通讯作者：
Junquera Escribano C
影响因子：
5.3
作者：
Kostin S
通讯作者：
Kostin S
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.