Whole-genome sequencing reveals Listeria monocytogenes diversity and allows identification of long-term persistent strains in Brazil.
Whole-genome sequencing reveals Listeria monocytogenes diversity and allows identification of long-term persistent strains in Brazil.
复制标题
DOI：
10.1111/1462-2920.14726
复制
发表时间：
2019-12
影响因子：
5.1
通讯作者：
Nero LA
中科院分区：
文献类型：
--
作者：
Camargo AC;Moura A;Avillan J;Herman N;McFarland AP;Sreevatsan S;Call DR;Woodward JJ;Lecuit M;Nero LA
全基因组测序（WGS）技术的进步已经记录了欧洲和北美主要食源性病原体单核细胞增生李斯特菌（Lm）的遗传多样性和流行病学，但有关南美的数据很少。在这里，我们研究了在巴西收集的这种主要食源性病原体的种群结构和遗传多样性。基于核心基因组多位点序列分型（cgMLST），来自谱系I（n = 22; 63%）和谱系II（n = 13; 37%）的分离物分布到10个不同的亚谱系（SL）中，代表31个新的cgMLST类型（CT）。最常见的SL为SL 9（n = 9; 26%）、SL 3（n = 6; 17%）以及SL 2和SL 218（n = 5; 14%）。属于CT L2-SL 9-ST 9-CT 4420和L1-SL 315-ST 520-CT 4429的分离株分别间隔3年和9年收集，揭示了Lm在巴西的长期持久性。60%的分离株（57%的SSI-1和3%的SSI-2）中存在与应激存活相关的遗传因子。LIPI-1、LIPI-3和LIPI-4的致病岛检出率分别为100%、43%和6%。在prfA和inlA毒力基因中检测到导致过早终止密码子的突变。这项研究是了解Lm在南美的基因组多样性和流行病学的重要贡献。此外，研究结果强调了使用WGS揭示Lm长期持续性的重要性。
Advances in whole-genome sequencing (WGS) technologies have documented genetic diversity and epidemiology of the major foodborne pathogen Listeria monocytogenes (Lm) in Europe and North America, but data concerning South America are scarce. Here, we examined the population structure and genetic diversity of this major foodborne pathogen collected in Brazil. Based on core genome multilocus sequence typing (cgMLST), isolates from lineages I (n = 22; 63%) and II (n = 13; 37%) were distributed into 10 different sublineages (SLs) and represented 31 new cgMLST types (CTs). The most prevalent SLs were SL9 (n = 9; 26%), SL3 (n = 6; 17%) and SL2 and SL218 (n = 5; 14%). Isolates belonging to CTs L2-SL9-ST9-CT4420 and L1-SL315-ST520-CT4429 were collected 3 and 9 years apart, respectively, revealing long-term persistence of Lm in Brazil. Genetic elements associated with stress survival were present in 60% of isolates (57% SSI-1 and 3% SSI-2). Pathogenic islands were present in 100% (LIPI-1), 43% (LIPI-3) and 6% (LIPI-4) of the isolates. Mutations leading to premature stop codons were detected in the prfA and inlA virulence genes. This study is an important contribution to understanding the genomic diversity and epidemiology of Lm in South America. In addition, the results highlight the importance of using WGS to reveal Lm long-term persistence.
登录
查看更多内容
影响因子：
56.3
作者：
de Noordhout, Charline Maertens;Devleesschauwer, Brecht;Angulo, Frederick J.;Verbeke, Geert;Haagsma, Juanita;Kirk, Martyn;Havelaar, Arie;Speybroeck, Niko
通讯作者：
Speybroeck, Niko
影响因子：
56.3
作者：
Charlier, Caroline;Perrodeau, Elodie;Lecuit, Marc
通讯作者：
Lecuit, Marc
影响因子：
11.8
作者：
Chenal-Francisque, Viviane;Lopez, Jodie;Brisse, Sylvain
通讯作者：
Brisse, Sylvain
DOI：
10.1590/s0037-86822006000100006
发表时间：
2006-02-01
影响因子：
2
作者：
Hofer, Ernesto;Reis, Cristhiane Moura Falavina dos;Hofer, Cristina Barroso
通讯作者：
Hofer, Cristina Barroso
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.