The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri.
The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri.
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ColRS 调节的膜蛋白基因 XAC1347 参与柑橘黄单胞菌的铜稳态和 hrp 基因表达
DOI：
10.3389/fmicb.2018.01171
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zou H
中科院分区：
文献类型：
--
作者：
Fan X;Guo J;Zhou Y;Zhuo T;Hu X;Zou H
柑橘黄单胞菌亚种。柑桔酸（Xcc）是柑桔溃疡病的主要致病因子。XAC1347基因在Xcc中编码一种保守的膜蛋白，是感染过程中产生毒力所必需的。然而，XAC1347介导的分子事件尚不清楚。在本研究中，我们报道了XAC1347基因受到双组分调节系统ColRS的正调控，是III型分泌系统功能所必需的。XAC1347基因的非极性缺失突变体导致植物中Hrp缺失表型和铜稳态降低。实时荧光定量PCR实验表明，XAC1347基因是铜离子诱导的。在XAC1347突变体中，hrpB1、hrcV、hrpF和hrpD6四个hrp操纵子的代表性基因表达水平降低，表明XAC1347参与了hrp基因的表达。
Xanthomonas citri subsp. citri (Xcc) is the major causal agent of citrus canker disease. The XAC1347 gene, which encodes a conserved membrane protein in Xcc, is required for virulence during infection. However, the molecular events mediated by XAC1347 remain unclear. In this study, we reported that XAC1347 gene is positively regulated by two component regulatory system ColRS and required for type III secretion system function. A non-polar deletion mutant of the XAC1347 gene resulted in a Hrp minus phenotype in plants and reduced copper homeostasis. Real-time PCR experiments indicated that XAC1347 gene is induced by copper ions. The expression levels of representative genes from four hrp operons, including hrpB1, hrcV, hrpF, and hrpD6, were reduced in XAC1347 mutant, indicating that XAC1347 is involved hrp gene expression.
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影响因子：
3.3
作者：
Carnielli, Carolina Moretto;Artier, Juliana;Marques Novo-Mansur, Maria Teresa
通讯作者：
Marques Novo-Mansur, Maria Teresa
影响因子：
3.4
作者：
Kar UK;Simonian M;Whitelegge JP
通讯作者：
Whitelegge JP
影响因子：
2.7
作者：
Dunger, G;Arabolaza, AL;Ottado, J
通讯作者：
Ottado, J
HrpD6 在调节米黄单胞菌 hrp-hrc-hpa 基因中的新调控作用。
影响因子：
3.5
作者：
Li, Yu-Rong;Zou, Hua-Song;Chen, Gong-You
通讯作者：
Chen, Gong-You
影响因子：
3.6
作者：
Horak, R;Ilves, H;Kivisaar, M
通讯作者：
Kivisaar, M