Identification and characterization of layer-specific differences in extraocular muscle m-bands.
Identification and characterization of layer-specific differences in extraocular muscle m-bands.
复制标题
眼外肌 m 带层特异性差异的识别和表征。
DOI：
10.1167/iovs.06-0701
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发表时间：
2007
影响因子：
4.4
通讯作者：
Khurana,TejvirS
中科院分区：
文献类型：
--
作者：
Wiesen,MartinHJ;Bogdanovich,Sasha;Agarkova,Irina;Perriard,Jean-Claude;Khurana,TejvirS
目的.目的：研究成年大鼠眼外肌眶层（OL）和球层（GL）中M带（或M线）的表达。半定量聚合酶链反应（PCR），定量（q）PCR，免疫组化和共聚焦显微镜分析的主要基因和蛋白质成分的表达的M带新鲜解剖和冷冻切片的眼外直肌（EOMs）和胫骨前肌（TA）。电子显微镜（EM）进行灌注固定的EOMs和TA肌肉在层的特定方式，以确定，表征和量化的M带表达的椎板特异性差异。这些研究表明，通过使用EM，半定量PCR，qPCR，免疫组织化学和共聚焦显微镜，在结构，mRNA和蛋白质水平上，M带及其主要成分myomesin 1（Myom 1）和myomesin 2（Myom 2或M蛋白）的表达存在EOM层特异性差异。通过使用基于EM的粗丝间距离和方差测量来量化粗丝晶格顺序的差异，并且发现TA> GL> OL。M带及其组分在EOMs中的表达模式为其同种异型和层特异性粘弹性质提供了机理上的见解。建模的差异表达的M-带之间的EOMs和TA预测弹性增加，但减少力和偏心收缩（ECC）介导的损害EOMs，并提出了一个潜在的机制，为临床节省的EOMs注意到杜氏肌营养不良症（DMD）。
purpose. To examine and characterize the expression of M-bands (or M-lines) in the orbital layer (OL) and global layer (GL) of adult rat extraocular muscles (EOMs).methods. Semiquantitative polymerase chain reaction (PCR), quantitative (q) PCR, immunohistochemistry, and confocal microscopy were used to analyze expression of the major gene and protein constituents of M-bands in freshly dissected and cryosectioned rectus extraocular muscles (EOMs) and tibialis anterior (TA) muscles. Electron microscopy (EM) was performed on perfusion-fixed EOMs and TA muscles in a layer-specific manner, to determine, characterize, and quantify laminar-specific differences in M-band expression.results. These studies demonstrate EOM layer-specific differences in the expression of M-bands and their major constituents, myomesin1 (Myom1) and myomesin2 (Myom2 or M-protein) at the structural, mRNA, and protein levels by using EM, semiquantitative PCR, qPCR, immunohistochemistry, and confocal microscopy. Differences in thick filament lattice order were quantified by using EM-based inter-thick-filament distance and variance measurements and were found to be TA> GL> OL.conclusions. The expression pattern of M-bands and their constituents in EOMs provides mechanistic insight for their allotypic and layer-specific viscoelastic properties. Modeling the differential expression of M-bands between EOMs and TA predicts increased elasticity but reduced force and eccentric contraction (ECC)-mediated damage in EOMs and suggests a potential mechanism for the clinical sparing of EOMs noted in Duchenne’s muscular dystrophy (DMD).
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肉毒杆菌引起的猴子眼睑肌肉的变化。
DOI：
10.1001/archopht.1991.01080030098047
发表时间：
1991
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Porter,JD;Strebeck,S;Capra,NF
通讯作者：
Capra,NF
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DOI：
--
发表时间：
2000
期刊：
Journal of Neurological Sciences
影响因子：
--
作者：
C. Niemann;T. Krag;T. Khurana
通讯作者：
T. Khurana
影响因子：
2.4
作者：
G. Ruskell;Inga‐Britt Kjellevold Haugen;J. R. Bruenech;F. van der Werf
通讯作者：
F. van der Werf
影响因子：
3.6
作者：
CARLSSON, E;THORNELL, LE
通讯作者：
THORNELL, LE
脊椎动物肌肉 M 区的三维结构。
影响因子：
5.6
作者：
P. Luther;J. Squire
通讯作者：
J. Squire