Interneuron‐specific Ca2+ Responses Linked to Metabotropic ‐and lonotropic Glutamate Receptors in Rat Hippocampal Slices
Interneuron‐specific Ca2+ Responses Linked to Metabotropic ‐and lonotropic Glutamate Receptors in Rat Hippocampal Slices
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大鼠海马切片中与代谢型和变向型谷氨酸受体相关的中间神经元特异性 Ca2+ 反应
DOI：
10.1111/j.1460-9568.1997.tb01520.x
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发表时间：
1997
影响因子：
3.4
通讯作者：
J. Lacaille
中科院分区：
文献类型：
--
作者：
L. Carmant;G. Woodhall;M. Ouardouz;R. Robitaille;J. Lacaille
在大鼠海马切片中，使用共聚焦显微镜和Ca 2+指示剂fluo 3-AM，在不同群体的CA 1中间神经元中检测谷氨酸介导的细胞内Ca 2+水平调节。基底层[方向肺泡层（OA）]和顶端层[放射层和腔隙分子层（R/LM）]树突层的中间神经元对谷氨酸的反应不均匀。在对照培养基中，OA中间神经元的反应主要是振荡的Ca 2+反应，其中包括一个大的Ca 2+瞬态和连续的较小的海拔。R/LM中间神经元主要以双相反应响应，其特征在于初始大瞬态和次级长时间升高。R/LM和OA中的其他中间神经元的Ca 2+水平瞬时升高。离子型谷氨酸受体拮抗剂（±）2-氨基-5-膦酰基戊酸和6-氰基-7-硝基-喹喔啉-2，3-二酮降低OA和R/LM细胞的峰值Ca 2+反应，并阻断R/LM中间神经元的双相反应。代谢型谷氨酸受体拮抗剂（RS）-α-甲基-4-羧基苯甘氨酸仅在OA中间神经元中降低峰值Ca 2+反应，并阻止振荡反应。在低Ca ~（2+）介质中，R/LM中间神经元的峰值反应降低，而OA中间神经元的峰值反应没有降低，振荡反应消失。离子型和代谢型受体拮抗剂的组合阻断了所有谷氨酸诱发的Ca 2+反应。因此，不同类型的谷氨酸受体的激活可能会在CA 1中间神经元的亚群中产生异质性Ca 2+信号。亲离子受体可能会在顶端树枝层的中间神经元中产生双相反应，而亲代谢受体和亲离子受体的联合激活可能会引发基底树枝层中间神经元的振荡反应。这些不均匀的Ca 2+反应表明，谷氨酸介导的Ca 2+过程和第二信使系统在海马中间神经元的亚群中不同，并表明中间神经元可能的突触后功能特化。
Glutamate‐mediated regulation of intracellular Ca2+ levels was examined in different populations of CA1 interneurons, using confocal microscopy and the Ca2+ indicator fluo 3‐AM in rat hippocampal slices. Interneurons in basal [stratum oriendalveus (OA)] and apical [strata radiatum and lacunosum‐moleculare (R/LM)] dendritic layers responded heterogeneously to glutamate. In control medium, OA interneurons responded mostly with oscillatory Ca2+ responses, which consisted of a large Ca2+ transient and successive smaller elevations. R/LM interneurons responded mostly with biphasic responses, characterized by an initial large transient and a secondary prolonged elevation. Other interneurons in both R/LM and OA responded with transient elevations in Ca2+ levels. lonotropic glutamate receptor antagonists (±)2‐amino‐5‐phosphonopentanoic acid and 6‐cyano‐7‐nitro‐quinoxaline‐2,3‐dione reduced peak Ca2+ responses in OA and R/LM cells, and blocked biphasic responses in R/LM interneurons. The metabotropic glutamate receptor antagonist (RS)‐α‐methyl‐4‐carboxyphenylglycine reduced peak Ca2+ responses only in OA interneurons, and prevented oscillatory responses. In low Ca2+ medium, peak responses were reduced in R/LM but not in OA interneurons, and oscillatory responses were absent. Combination of ionotropic and metabotropic receptor antagonists blocked all glutamate‐evoked Ca2+ responses. Activation of different types of glutamate receptors may thus produce heterogeneous Ca2+ signals in subpopulations of CA1 interneurons. lonotropic receptors may generate biphasic responses in interneurons in apical dendritic layers, whereas combined activation of metabotropic and ionotropic receptors may trigger oscillatory responses in interneurons of basal dendritic layers. These heterogeneous Ca2+ responses indicate that glutamate‐mediated Ca2+ processes and second messenger systems differ in subpopulations of hippocampal interneurons and suggest possible postsynaptic functional specialization of interneurons.
两种不同的使君子酸受体在体外调节海马神经元的 Ca2 稳态。
影响因子：
3.6
作者：
Murphy,SN;Miller,RJ
通讯作者：
Miller,RJ
影响因子：
5.5
作者：
SAH, P;HESTRIN, S;NICOLL, RA
通讯作者：
NICOLL, RA
红藻氨酸诱导的癫痫持续状态对成熟和未成熟动物中肌醇三磷酸和癫痫引起的脑损伤的影响。
DOI：
10.1016/0165-3806(95)00110-y
发表时间：
1995
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Carmant,L;Liu,Z;Werner,SJ;Mikati,MA;Holmes,GL
通讯作者：
Holmes,GL