Synthesis of Cysteinyl-tRNACys by A Prolyl-tRNA Synthetase
Synthesis of Cysteinyl-tRNACys by A Prolyl-tRNA Synthetase
复制标题
脯氨酰-tRNA 合成酶合成半胱氨酰-tRNACys
作者：
Chun;Ya
在缺乏典型半胱氨酸- trna合成酶（cyysrs）的生物体中，半胱氨酸- trnacys是如何合成的问题是理解蛋白质合成的一个重要开放性问题。广泛的生物体的脯氨酸- trna合成酶（pros）具有不经编辑就能误激活半胱氨酸的能力。这就提出了一个问题，即错误激活的半胱氨酸是否可以被充电到tRNACys上以合成正确匹配的cys-tRNACys，这可能在缺乏常规CysRS的生物体中作为一种选择。尽管进行了密集的搜索，但没有发现这种活动。本研究表明耐辐射细菌耐辐射球菌（Deinococcus radiodurans）的pros具有将半胱氨酸装载到tRNACys上的能力，并且无需编辑即可稳定地保留同源对cys-tRNACys。然而，耐辐射球菌的半胱氨酸化并不是合成cyss - trnacys的主要途径，因为生物体编码常规的CysRS。尽管如此，体外合成cys-tRNACys是很重要的，它不像以前记录的jannaschii Methanocaldococcus ProRS错充，后者合成cys-tRNAPro而不是cys-tRNACys。我们认为，在其他因素存在的情况下，D. radiodruans ProRS的半胱氨酸化活性可能为缺乏常规CysRS的生物体在体内合成半胱氨酸- trnacys提供了一种选择。
The question of how cysteinyl-tRNACys is synthesized in organisms that lack a canonical cysteinyl-tRNA synthetase (CysRS) is an important open question in understanding protein synthesis. The prolyl-tRNA synthetase (ProRS) of wide ranging organisms has the ability to mis-activate cysteine without editing. This raises the question of whether the mis-activated cysteine can be charged to tRNACys to synthesize the correctly matched cys-tRNACys, which may serve as an option in organisms that lack the conventional CysRS. Despite intense searches, such an activity has not been found. Here we show that the ProRS of the radiation-resistant bacterium Deinococcus radiodurans has the ability to charge cysteine to tRNACys and to retain the cognate pair cys-tRNACys stably without editing. The cysteinylation by D. radiodurans ProRS is not the major route for synthesis of cys-tRNACys, however, because the organism encodes the conventional CysRS. Nonetheless, the synthesis of cys-tRNACys in vitro is important and it is unlike previously documented mis-charging of Methanocaldococcus jannaschii ProRS, which synthesizes cys-tRNAPro but not cys-tRNACys. We suggest that the cysteinylation activity of D. radiodruans ProRS may, in the presence of additional factors, offer one option to organisms that lack the conventional CysRS to synthesize cysteinyl-tRNACys in vivo.
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影响因子：
56.9
作者：
White, O;Eisen, JA;Fraser, CM
通讯作者：
Fraser, CM
大肠杆菌半胱氨酰-tRNA 合成酶对 tRNA(Cys) 的识别。
影响因子：
2.9
作者：
Komatsoulis,GA;Abelson,J
通讯作者：
Abelson,J
DOI：
10.1073/pnas.95.23.13495
发表时间：
1998-11-10
影响因子：
11.1
作者：
Lipman, RSA;Hou, YM
通讯作者：
Hou, YM
氨酰-tRNA 合成酶的高度分化金属中心可区分氨基酸。
DOI：
10.1021/bi034812u
发表时间：
2003
期刊：
Biochemistry.
影响因子：
--
作者：
Zhang,Chun-Mei;Perona,JohnJ;Hou,Ya-Ming
通讯作者：
Hou,Ya-Ming
DOI：
10.1073/pnas.97.16.8916
发表时间：
2000-08-01
影响因子：
11.1
作者：
Beuning, PJ;Musier-Forsyth, K
通讯作者：
Musier-Forsyth, K