Failed identification of Candida vulturna using the updated Vitek 2 yeast identification system, version 9.02 and CHROMagar Candida Plus.
Failed identification of Candida vulturna using the updated Vitek 2 yeast identification system, version 9.02 and CHROMagar Candida Plus.
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DOI：
10.1016/j.nmni.2022.101012
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发表时间：
2022-07
影响因子：
4
通讯作者：
Sevillano, G.
中科院分区：
文献类型：
--
作者：
Zurita, J.;Paz y Mino, A.;Solis, M. B.;Sevillano, G.
我们描述了一例69岁的男性糖尿病患者。2021年8月25日，我们实验室收到了一份足部伤口标本进行培养。样品放置在CHRO-Magar Candida Plus，Sabouraud琼脂和Mycoel琼脂上。使用VITEK 2自动识别系统(BioMérieux)进行识别，并根据制造商的说明，使用VITEK 2识别系统版本09.01的软件进行识别。获得低分辨(LD)结果时，不能区分具有50%同源性的金黄色葡萄球菌、多布沙穆隆氏葡萄球菌。用VITEK系统对分离株进行了两次分析，与金黄色葡萄球菌的同源性分别为93%和91%。将该酵母菌放入特效培养基ChROMagar Candida Plus中，以区分金黄色念珠菌和其他念珠菌，其特异性和敏感度为100%[2]。金黄色葡萄球菌具有典型的形态特征(图1)[2]，用大亚基RNA基因(d1-d2区)及其区域进行鉴定。用MEGAX软件对D1D2序列进行最大似然模型进化分析。测序结果表明，ITS和D1/D2的同源性分别为99.87%和100%。系统发育分析表明，该菌与病人(ZZ3048)的序列组成了一个单系分支，其序列的离散率为0%，表明该菌的序列与所分析的标本完全一致，被认为是一个物种
We describe a case of 69-year-old man that is diabetic. On August 25, 2021, we received in our laboratory a sample from foot wound for culture. The sample was placed on CHRO-Magar Candida Plus, Sabouraud agar, and Mycosel agar. The Vitek 2 automated identification system (bioMérieux) was used to identification and was performed according to the manufacturer’s instructions, using the software of the Vitek 2 identification system version 09.01. Low-discrimination (LD) results were obtained with an inability to distinguish between C. auris, C. duobushaemulonii, with a 50% identity percentage. The isolated was again analyzed using the Vitek system on two occasions with 93% and 91% of identity for C. auris, respectively. This yeast was placed to CHROMagar Candida Plus, specific medium to differentiate C. auris from other Candida species, with a specificity and sensitivity of 100%[2]. The typical appearance of C. auris was present (Fig. 1)[2].The isolate was identified using large-subunit RNA gene (D1-D2 region) and ITS region. The maximum likelihood model was used for phylogenetic analysis with the D1/D2 sequences using MEGAX. The sequencing result showed an identity percentage of 99.87% and 100% using ITS and D1/D2 for C. vulturna, respectively. Phylogenetic analysis showed a monophyletic clade composed of Candida vulturna that includes the sequence obtained from the patient (ZZ3048), with a divergence percentage of 0%, indicating that the C. vulturna sequences and the analyzed sample are identical, and was considered as a species
影响因子：
9.4
作者：
Simon, Loic;Ughetto, Estelle;Ruimy, Raymond
通讯作者：
Ruimy, Raymond
影响因子：
2.9
作者：
Borman, Andrew M.;Fraser, Mark;Johnson, Elizabeth M.
通讯作者：
Johnson, Elizabeth M.