Human basophils express amphiregulin in response to T cell-derived IL-3.
Human basophils express amphiregulin in response to T cell-derived IL-3.
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DOI：
10.1016/j.jaci.2010.08.040
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发表时间：
2010-12
影响因子：
14.2
通讯作者：
Mosmann, Tim R.
中科院分区：
文献类型：
--
作者：
Qi, Yilin;Operario, Darwin J.;Oberholzer, Christopher M.;Kobie, James J.;Looney, R. John;Georas, Steve N.;Mosmann, Tim R.
两性调节蛋白是表皮生长因子家族的成员之一，由活化的小鼠Th2细胞表达。由小鼠造血细胞产生的两性调节素有助于通过2型效应器反应消除线虫感染。鉴定表达双调蛋白的人外周血细胞群。细胞表面标志物的流式细胞仪检测和组织学染色鉴定表达双调蛋白的细胞。用酶免疫法测定培养上清液中组胺和两调节素的含量。用实时荧光定量聚合酶链式反应检测mRNA的表达。抗CD3+抗CD28抗体刺激人外周血单核细胞可诱导非T细胞群体表达双调节蛋白基因和蛋白。从CD203c和CD123(IL-3受体α链)的形态和表达来看，产生双调节蛋白的细胞是嗜碱性细胞。被激活的小鼠嗜碱性粒细胞也会产生双调蛋白。嗜碱性粒细胞在抗TCR刺激下表达双调蛋白需要T细胞产生IL-3，而IL-3单独通过纯化的嗜碱性粒细胞诱导高水平的双调蛋白表达。当IL-3刺激人嗜碱性粒细胞时，AmphiRegin的表达水平远远高于IgE交联刺激的水平，而IL-4的表达和组胺释放则相反。IL-3刺激的嗜碱性粒细胞也表达肝素结合的表皮生长因子样生长因子。与过敏性或非过敏性对照相比，哮喘受试者的PBMC含有显著更多的能够产生双调节蛋白的嗜碱性粒细胞。IL-3可以诱导嗜碱性粒细胞表达高水平的双调节蛋白，这可能在哮喘等2型免疫反应中促进组织重塑。
Amphiregulin, a member of the Epidermal Growth Factor family, is expressed by activated mouse Th2 cells. Amphiregulin produced by mouse hematopoietic cells contributes to the elimination of a nematode infection by a Type 2 effector response. To identify the human peripheral blood cell population expressing amphiregulin. Amphiregulin-expressing cells were identified by flow cytometry of cell surface markers and histological staining. Histamine and amphiregulin in supernatants were measured by enzyme immunoassay. Quantitative real-time PCR was used to measure mRNA expression. Stimulation of human peripheral blood mononuclear cells by anti-CD3 + anti-CD28 antibodies induced expression of amphiregulin mRNA and protein by a non-T cell population. The amphiregulin-producing cells were basophils, as judged by morphology and expression of CD203c and CD123 (IL-3 receptor alpha chain). Activated mouse basophils also produced amphiregulin. Amphiregulin expression by basophils in response to anti-TCR stimulation required IL-3 produced by T cells, and IL-3 alone induced high levels of amphiregulin expression by purified basophils. Amphiregulin was expressed at much higher levels when human basophils were stimulated by IL-3 than by IgE cross-linking, whereas the opposite was true for IL-4 expression and histamine release. Heparin-binding Epidermal Growth Factor-like growth factor was also expressed by IL-3-stimulated human basophils. PBMC from asthmatic human subjects contained significantly higher numbers of basophils able to produce amphiregulin, compared to allergic or non-allergic controls. IL-3 can induce basophils to express high levels of amphiregulin, which may contribute to tissue remodeling during type 2 immune responses such as asthma.
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.4
作者：
Dzionek, A;Fuchs, A;Schmitz, J
通讯作者：
Schmitz, J
DOI：
10.1084/jem.193.2.255
发表时间：
2001-01-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hirai H;Tanaka K;Yoshie O;Ogawa K;Kenmotsu K;Takamori Y;Ichimasa M;Sugamura K;Nakamura M;Takano S;Nagata K
通讯作者：
Nagata K
白介素3（IL-3）引发的人外周血嗜碱性嗜碱性粒细胞对免疫球蛋白E受体刺激产生IL-4。
DOI：
10.1084/jem.177.3.605
发表时间：
1993-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Brunner T;Heusser CH;Dahinden CA
通讯作者：
Dahinden CA
DOI：
10.4049/jimmunol.0801782
发表时间：
2009-02-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schroeder JT;Chichester KL;Bieneman AP
通讯作者：
Bieneman AP