Photoperiodic effects on seasonal physiology, reproductive status and hypothalamic gene expression in young male F344 rats.
Photoperiodic effects on seasonal physiology, reproductive status and hypothalamic gene expression in young male F344 rats.
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DOI：
10.1111/jne.12241
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发表时间：
2015-02
影响因子：
3.2
通讯作者：
Helfer G
中科院分区：
文献类型：
--
作者：
Tavolaro FM;Thomson LM;Ross AW;Morgan PJ;Helfer G
季节性或对光周期敏感的动物通过生理(生长、摄食量和生殖状态)和行为的变化来响应日长的变化，以适应可预测的气候年度变化。通常，不同种类的仓鼠、田鼠和绵羊是光周期症研究最多的动物模型。尽管实验室老鼠通常被认为是非光周期的，但有一种老鼠品系，即近交系Fischer 344(F344)大鼠，已被证明对日光暴露的长度敏感，它会根据季节改变其生理表型和繁殖状态。本研究旨在更好地了解F344大鼠的光周期反应的性质。我们研究了五种不同的光周期对生理和神经内分泌反应的影响。将青年雄性F344大鼠置于光照8h/d到16h/d的光照条件下，比较体重，包括脂肪和瘦肉质量、摄食量、睾丸重量和下丘脑基因表达。我们发现，与≥光周期为10小时/天的大鼠相比，处于≤光周期下的大鼠显示出更多的生长和食物摄入量。磁共振成像分析证实，这些变化主要是瘦体重变化的结果。同样的模式在生殖状态下也很明显，在光照12小时/天的情况下，≥的光周期中对睾丸重量更高。伴随着生理状态的变化，下丘脑甲状腺激素(Dio2和Dio3)、维甲酸(Crabp1和Stra6)以及Wnt/β-catenin信号基因(SFRP2和Mfrp)也发生了重大变化。我们的数据表明，幼年雄性F344大鼠的神经内分泌系统将每天12小时的光周期解释为刺激性光周期。
Seasonal or photoperiodically sensitive animals respond to altered day length with changes in physiology (growth, food intake and reproductive status) and behaviour to adapt to predictable yearly changes in the climate. Typically, different species of hamsters, voles and sheep are the most studied animal models of photoperiodism. Although laboratory rats are generally considered nonphotoperiodic, one rat strain, the inbred Fischer 344 (F344) rat, has been shown to be sensitive to the length of daylight exposure by changing its physiological phenotype and reproductive status according to the season. The present study aimed to better understand the nature of the photoperiodic response in the F344 rat. We examined the effects of five different photoperiods on the physiological and neuroendocrine responses. Young male F344 rats were held under light schedules ranging from 8 h of light/day to 16 h of light/day, and then body weight, including fat and lean mass, food intake, testes weights and hypothalamic gene expression were compared. We found that rats held under photoperiods of ≥ 12 h of light/day showed increased growth and food intake relative to rats held under photoperiods of ≤ 10 h of light/day. Magnetic resonance imaging analysis confirmed that these changes were mainly the result of a change in lean body mass. The same pattern was evident for reproductive status, with higher paired testes weight in photoperiods of ≥ 12 h of light/day. Accompanying the changes in physiological status were major changes in hypothalamic thyroid hormone (Dio2 and Dio3), retinoic acid (Crabp1 and Stra6) and Wnt/β-Catenin signalling genes (sFrp2 and Mfrp). Our data demonstrate that a photoperiod schedule of 12 h of light/day is interpreted as a stimulatory photoperiod by the neuroendocrine system of young male F344 rats.
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影响因子：
4.3
作者：
Nakane Y;Yoshimura T
通讯作者：
Yoshimura T
影响因子：
3.7
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Otsuka T;Goto M;Kawai M;Togo Y;Sato K;Katoh K;Furuse M;Yasuo S
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Yasuo S
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Lobley, Gerald E.;Bremner, David M.;Maloney, Christopher
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Maloney, Christopher
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Barrett, Perry;Ebling, Francis J. P.;Morgan, Peter J.
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Morgan, Peter J.
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3.6
作者：
Heideman, PD;Bierl, CK;Galvez, ME
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