Divergent gene expression responses to complicated grief and non-complicated grief.
Divergent gene expression responses to complicated grief and non-complicated grief.
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DOI：
10.1016/j.bbi.2013.12.017
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发表时间：
2014-03
影响因子：
15.1
通讯作者：
Cole, Steven W.
中科院分区：
文献类型：
--
作者：
O'Connor, Mary-Frances;Schultze-Florey, Christian R.;Irwin, Michael R.;Arevalo, Jesusa M. G.;Cole, Steven W.
关键词：
“寡妇效应”（即，最近丧亲配偶的发病率/死亡率）可能与免疫功能的变化有关，但关于丧亲之痛对免疫细胞基因转录的影响知之甚少。本研究探讨了复杂性悲伤和非复杂性悲伤对丧亲之痛的反应如何不同地影响白细胞基因表达。对63名老年人完成了全基因组转录谱分析和生物信息学分析。其中36人平均在2年前失去了配偶/伴侣，27人是非丧偶的已婚对照组。12名死者家属符合复杂悲伤的标准。与无丧亲之痛的对照组相比，丧亲之痛（复杂性悲伤和非复杂性悲伤）与参与一般免疫激活的基因表达上调和参与B淋巴细胞反应的基因选择性下调有关。然而，复杂的悲伤和非复杂的悲伤显着不同的I型干扰素相关的转录本的表达，与非复杂的悲伤科目显示出实质性的上调相对于nonbeared控制和复杂的悲伤科目显示出实质性的下调。丧亲显著调节免疫功能基因表达。与丧亲之痛相关的痛苦程度（即，复杂性悲伤与非复杂性悲伤）与参与先天性抗病毒反应的转录因子激活和基因表达的差异模式有关。这些发现为理解丧亲之痛对健康的影响提供了一个分子框架，也为了解对个体对损失的心理反应最敏感的特定基因模块提供了新的见解。
The “widowhood effect” (i.e., morbidity/mortality in recently bereaved spouses) may be related to changes in immune function, but little is known about the impact of bereavement on gene transcription in immune cells. This study examined how Complicated Grief and Non-complicated Grief responses to bereavement differentially affect leukocyte gene expression. Genome-wide transcriptional profiling and bioinformatic analyses were completed on 63 older adults. Thirty-six of them had lost their spouse/partner on average 2 years ago, and 27 were nonbereaved, married controls. Twelve of the bereaved participants met criteria for Complicated Grief. Compared to nonbereaved controls, bereavement (both Complicated Grief and Non-complicated Grief) was associated with upregulated expression of genes involved in general immunologic activation and a selective downregulation of genes involved in B lymphocyte responses. However, Complicated Grief and Non-complicated Grief differed markedly in their expression of Type I interferon-related transcripts, with Non-complicated Grief subjects showing substantial upregulation relative to nonbereaved controls and Complicated Grief subjects showing substantial downregulation. Bereavement significantly modulates immune function gene expression. The magnitude of bereavement-related distress (i.e., Complicated Grief vs. Non-complicated Grief) is linked to differential patterns of transcription factor activation and gene expression involved in innate antiviral responses. These findings provide a molecular framework for understanding the health effects of bereavement, as well as new insights into the particular gene modules that are most sensitive to the individual's psychological response to loss.
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影响因子：
5
作者：
COHEN, S;KAMARCK, T;MERMELSTEIN, R
通讯作者：
MERMELSTEIN, R
影响因子：
5.4
作者：
Boyle, Paul J.;Feng, Zhiqiang;Raab, Gillian M.
通讯作者：
Raab, Gillian M.
影响因子：
5.8
作者：
Cole, SW;Yan, W;Zack, JA
通讯作者：
Zack, JA
DOI：
10.1073/pnas.1531903100
发表时间：
2003-07-22
影响因子：
11.1
作者：
Kiecolt-Glaser, JK;Preacher, KJ;Glaser, R
通讯作者：
Glaser, R
DOI：
10.1073/pnas.0911515107
发表时间：
2010-03-23
影响因子：
11.1
作者：
Cole, Steven W.;Arevalo, Jesusa M. G.;Seeman, Teresa E.
通讯作者：
Seeman, Teresa E.