Methods to mechanically perturb and characterize GUV-based minimal cell models.
Methods to mechanically perturb and characterize GUV-based minimal cell models.
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DOI：
10.1016/j.csbj.2022.12.025
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发表时间：
2023
影响因子：
6
通讯作者：
Liu, Allen P.
中科院分区：
文献类型：
--
作者：
Wubshet, Nadab H.;Liu, Allen P.
关键词：
细胞通过主要由磷脂和膜蛋白组成的活性边界屏蔽细胞器和胞质溶胶，但允许细胞内和细胞外环境之间的通信。通常称为巨单层囊泡（GUV）的微囊大小的脂质体隔室用于模拟细胞膜并将生物材料和过程封装在细胞样限制中。在自下而上合成生物学领域，许多人已经利用GUV作为底物来研究各种生物过程，例如蛋白质-脂质相互作用、细胞骨架组装和蛋白质合成的动力学。与细胞一样，理想的是GUV也具有机械耐用性，并且能够在内外环境发生变化时保持完整。因此，研究已经证明了通过调节膜组成和内腔材料性质来调节GUV的机械性质的方法。在这种情况下，已经开发了许多不同的方法来测试GUV的机械性能。在这篇评论中，我们将调查各种扰动技术的机械特性GUV。
Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment. Micron-sized liposome compartments commonly known as giant unilamellar vesicles (GUVs) are used to model the cell membrane and encapsulate biological materials and processes in a cell-like confinement. In the field of bottom-up synthetic biology, many have utilized GUVs as substrates to study various biological processes such as protein-lipid interactions, cytoskeletal assembly, and dynamics of protein synthesis. Like cells, it is ideal that GUVs are also mechanically durable and able to stay intact when the inner and outer environment changes. As a result, studies have demonstrated approaches to tune the mechanical properties of GUVs by modulating membrane composition and lumenal material property. In this context, there have been many different methods developed to test the mechanical properties of GUVs. In this review, we will survey various perturbation techniques employed to mechanically characterize GUVs.
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影响因子：
5
作者：
Bashirzadeh Y;Wubshet NH;Liu AP
通讯作者：
Liu AP
影响因子：
3.4
作者：
Aranda, Said;Riske, Karin A.;Dimova, Rumiana
通讯作者：
Dimova, Rumiana
影响因子：
2.6
作者：
Brungs, Sonja;Hauslage, Jens;Anken, Ralf
通讯作者：
Anken, Ralf
DOI：
10.1002/cphc.201700050
发表时间：
2017-06-20
期刊：
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子：
--
作者：
Chaudhary H;Subramaniam V;Claessens MMAE
通讯作者：
Claessens MMAE
影响因子：
64.8
作者：
ASHKIN, A;DZIEDZIC, JM;YAMANE, T
通讯作者：
YAMANE, T