Nanoscale imaging by superresolution fluorescence microscopy and its emerging applications in biomedical research.
Nanoscale imaging by superresolution fluorescence microscopy and its emerging applications in biomedical research.
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超分辨率荧光显微镜的纳米级成像及其在生物医学研究中的新兴应用。
DOI：
10.1615/critrevbiomedeng.2014010448
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发表时间：
2013
影响因子：
--
通讯作者：
P. Kanchanawong
中科院分区：
文献类型：
--
作者：
Cristina Bertocchi;Wah Ing Goh;Zhen Zhang;P. Kanchanawong
超分辨率显微镜是一种光学显微镜方法，其目的是超越衍射所施加的分辨率限制，近年来一直处于成像技术创新的最前沿。通过利用荧光团生物物理学、荧光蛋白工程、光学和图像处理的进步，通过3种主要方法在提高成像分辨率方面取得了快速进展：结构化照明显微镜、受激发射耗尽显微镜和单分子定位显微镜。从~250 nm的衍射极限分辨率，现在可以获得超过一个数量级的改进，下降到~10 nm，集中在细胞的大分子结构单元的尺寸尺度上。这开辟了直接可视化分子尺度结构和生物结构中对健康和疾病至关重要的特定蛋白质相互作用的可能性。在这里，超分辨显微镜在2-和3-维成像的理论基础和实际考虑进行了讨论，沿着他们最近的应用在解决生物问题。
Superresolution microscopy, an ensemble of light microscopy methods developed with the aim of surpassing the resolution limit imposed by diffraction, has been at the forefront of imaging technology innovations in recent years. By harnessing advances in fluorophore photophysics, fluorescent protein engineering, optics, and image processing, rapid strides have been made in enhancing imaging resolution via 3 major approaches: structured illumination microscopy, stimulated emission depletion microscopy, and single-molecule localization microscopy. From a diffraction-limited resolution of ~250 nm, an improvement of more than an order of magnitude down to ~10 nm can now be attained, converging upon the size scale of the macromolecular building blocks of cells. This opens up the possibility of direct visualization of molecular-scale architecture and interactions of specific proteins in biological structures that are important to health and disease. Here, theoretical foundations and practical considerations of superresolution microscopy in 2- and 3-dimensional imaging are discussed, along with their recent applications in addressing biological questions.
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影响因子：
15
作者：
Lee, Hsiao-lu D.;Lord, Samuel J.;Iwanaga, Shigeki;Zhan, Ke;Xie, Hexin;Williams, Jarrod C.;Wang, Hui;Bowman, Grant R.;Goley, Erin D.;Shapiro, Lucy;Twieg, Robert J.;Rao, Jianghong;Moerner, W. E.
通讯作者：
Moerner, W. E.
影响因子：
3.4
作者：
Thompson, RE;Larson, DR;Webb, WW
通讯作者：
Webb, WW
影响因子：
56.9
作者：
Schermelleh, Lothar;Carlton, Peter M.;Sedat, John W.
通讯作者：
Sedat, John W.
DOI：
10.1002/cphc.201100686
发表时间：
2012-03
期刊：
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子：
--
作者：
Biteen JS;Goley ED;Shapiro L;Moerner WE
通讯作者：
Moerner WE
影响因子：
3.4
作者：
KAO, HP;VERKMAN, AS
通讯作者：
VERKMAN, AS