cGMP stimulates sodium and chloride currents in rat tracheal airway epithelia.
cGMP stimulates sodium and chloride currents in rat tracheal airway epithelia.
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cGMP 刺激大鼠气管上皮细胞中的钠流和氯流。
DOI：
10.1152/ajpcell.1997.272.3.c911
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Guggino,SE
中科院分区：
文献类型：
--
作者：
Schwiebert,EM;Potter,ED;Hwang,TH;Woo,JS;Ding,C;Qiu,W;Guggino,WB;Levine,MA;Guggino,SE
To test the hypothesis that guanosine 3',5'-cyclic monophosphate (cGMP) regulates ion transport in airway epithelial cells, we measured short-circuit current (I(sc)) and (22)Na+ fluxes in primary cultured rat tracheal epithelial cells. In Cl- -containing Ringer solution, I(sc) was increased by approximately 17 microA/cm2 after application of 1 mM 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), whereas, in Cl- -free solutions, the Na+ -mediated component was approximately 5 microA/cm2, suggesting a cGMP stimulation of Cl-secretory current and a smaller Na+ absorptive current. Inward and net mucosal-to-serosal (22)Na+ flux was doubled in the presence of 2 mM 8-BrcGMP. To determine whether nucleotide-gated channels play a role in this transepithelial Na+ absorption, blockers of nucleotide-gated cation channels were used to inhibit I(sc). The cGMP-stimulated Na+-mediated I(sc) was blocked by as little as 500 nM dichlorobenzamil or 50 microM L-cis-diltiazem, which are known blockers for cyclic nucleotide-gated cation channels. These agents also blocked the basal (non-cGMP-stimulated) current when measured in the presence of 10 microM amiloride, which blocks current through 5-pS amiloride-sensitive Na+ channels. To document whether the distribution of nucleotide-gated nonselective cation channels was consistent with a role in airway epithelial transport, in situ hybridization was performed. In situ hybridization of mRNA encoding for nucleotide-gated cation channels was found in epithelial cell layers of rat trachea, bronchi, bronchioles, and alveolar cells but not in smooth muscle layers or tracheal cartilage. Reverse transcriptase-polymerase chain reaction, restriction enzyme analysis, and sequencing of the cDNA transcribed from mRNA of whole lung and tracheal epithelial cells indicate that a channel highly homologous to the retinal nucleotide-gated nonselective cation channel (CNG1) is present. Thus these data, along with evidence supporting the existence of signal transduction pathways elevating intracellular levels of cGMP, indicate that cGMP regulates transepithelial ion transport in lung epithelial tissues.
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影响因子：
--
作者：
Meiqiu Lin;K. MacLeod;S. Guggino
通讯作者：
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影响因子：
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作者：
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10.1073/pnas.83.8.2767
发表时间：
1986-04-01
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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通讯作者：
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